﻿56 Arthur and Fromme: A new Endophyllum 



botanical department of the Purdue Experiment Station for the 

 past ten years and during a considerable part of this period infected 

 plants of Callirhoe involucrata have been grown in the experimental 

 garden at Lafayette. The probable Endophyllum character of 

 the form was recognized at the first from certain features in the 

 structure of the sorus, the perennial mycelium, and the failure of 

 field studies to disclose any likely alternate stage. In order to test 

 this assumption it was necessary to have material near at hand for 

 germination and infection studies and Mr. E. Bartholomew of 

 Stockton, Kansas, very kindly sent infected Callirhoe plants for 

 this purpose. The first consignment of these plants was received 

 in 1904 and they were subsequently grown in the garden three or 

 four years, producing aecia each year. Germination tests of the 

 spores made by the senior writer in 1907 disclosed only non- 

 septate germ tubes (Text fig. i) and the conclusion was reached 



cell. After a drai 



that the spores were true aeciospores and the structure an aecium 

 in spite of the inferential evidence to the contrary. We now know 

 that the method of germination commonly employed in such 

 tests, the hanging drop of the Van Tieghem cell, was responsible 

 for the failure to discover the true character of the germination. 

 Early in the present year of 1914 Mr. Bartholomew sent 

 another shipment of infected plants of Callirhoe involucrata and 

 germination tests were again instituted. The method of germina- 

 tion employed by Kunkel, sowing on the surface of a non-nutrient 

 agar, was used with complete success. Promycelia and basidio- 

 spores were produced in abundance in the first test and in the 

 score or more of subsequent tests that were made, proving con- 

 clusively that the spores are in reality teliospores. 



