142 ANDERSON: EMBRYOGENY OF MARTYNIA LOUISIANA 
In as muchas no morphological work seemed to have been 
done on this family, a study of Martynia louisiana and Martynia 
fragrans was undertaken, the results of which are given in the 
' following pages. While the study was based mainly on Martynia 
louisiana no structural differences between the two species were 
observed. 
MATERIAL AND METHODS 
The material used for this study was collected from plants 
of Martynia louisiana and Martynia fragrans grown in the field, 
in Montgomery County, Indiana. Collections were usually made 
during the month of August, when the plants were most vigorous. 
The apices of racemes, small flowers and stamens, pistils and 
young fruits in various stages of development were killed and 
fixed in a I per cent chromo-acetic acid or in chrom-osmic-acetic 
acid one to two hours and then in chromo-acetic acid twenty-four 
to thirty-six hours, washed, dehydrated, and embedded through 
chloroform. To determine the time necessary for the pollen 
tube to reach the egg, pollination was controlled, and, beginning 
at one hour after the application of the pollen, collections were 
made at intervals of one hour for fourteen consecutive hours. 
These collections were of whole pistils which were killed and 
fixed in chrom-osmic-acetic acid one to two hours and then in 
chromo-acetic acid thirty-six hours. Beginning thirty hours 
after pollination, collections of pistils and young fruits were 
made at intervals of six hours for two hundred and four hours. 
Sections were cut 4-10 wu, most of them 5 u thick; those of whole 
pistils were longitudina] and 4-5 y. in thickness. Both modified 
triple and Heidenhain’s iron-alum-haematoxylin were used for 
staining, the former being more satisfactory. 
THE DEVELOPMENT OF THE FLOWER 
As in most inflorescences, the flower of Martynia louisiana 
arises as a lateral branch (Fic. 1, X) in the axil of a 
bract (Fic. 1, B).. The first bract arises as a lateral papilla 
near the apex of the main axis of the raceme (Fic. 2, B’). 
This bract grows rapidly and about the time its tip reaches 
‘the apex of the raceme, a rather broad protuberance appears 
in the axil (Fic. 1, X). With further growth of the axis of the 
raceme other bracts appear, and in the axils of these the primordia 
of other flowers develop (Fic. 4, X). As the rudiment of 
