42 Journal of the Mitchell Society [August 



together and to the slide were obtained. These died within a day. It 

 should be added, however, that no special precautions were taken 

 against bacterial growth. 



Thirty-four pieces, 1 to 3 but mostly 2 mm. long, of fresh stolon 

 M'ere cut July 7 (1910). A considerable number of these pieces within 

 two days (July 9) developed a terminal or lateral bud, into which in 

 a number of instances the stolon two days later (July 11) had been 

 largely absorbed, as has been recently described by Huxley. 



Of great interest is the question how far reduction may be made 

 to go in these animals with retention of regenerative power. Employ- 

 ing the simple method by which I obtained in sponges gemmule-like 

 masses of regenerative tissue scattered through the dead body (Science, 

 June 7, 1907), I allowed a dozen Peropliora to die slowly in aquaria 

 in the hope that viable masses might be formed. All but one died 

 completely. But in the case of this one within the tunic and debris of 

 the original animal, a week after the inception of the experipaent, a 

 conspicuous mass of live tissue remained. This was of a bright green- 

 ish yellow color, apparently solid, irregularly lobed, and about 0.350 

 mm. in total diameter, the width of the lobes 0.220 mm. to 0.090 mm. 

 It would be of interest to work out the origin and test the regenerative 

 Gesellsch. Naturf. Freunde zu Berlin, No. 6, 1907) on the formation 

 of winter buds in ClavelUna probably have a bearing in this con- 

 nection. 



Chapel Hill, N. C. 



