58 Journal of the Mitchell Society [August 



used in our experiment analyzed 8.41% of protein. Likewise, the 

 Silver King corn planted in West Virginia and in North Carolina in 

 1922 showed a different analysis from that reported in paper I. The 

 corn used contained 9.07% of protein. More careful analysis of the 

 low protein corn planted in West Virginia gave 6.82% of protein. 



Our investigation in 1922 showed that the Silver King corn grown 

 in West Virginia, that analyzed 8.5% protein, when grown in North 

 Carolina, gave only 7.7% protein. Since our analysis of this corn, 

 we learned that it was planted late in the season, and was probably 

 not well matured. The ears were small, and the grains shriveled. We 

 are compelled to withhold any conclusions as to the effect of climate 

 on this reduction of protein, since the analysis of immature corn 

 shown in the tables, indicates a decided diminution in nitrogen. 



Results of Further Investigation of the Variation of Protein 



We have continued our investigation of variation in protein of 

 corn, by making analyses of the Silver King variety grown in West 

 Virginia and in North Carolina in 1922. The seed was grown in West 

 Virginia in 1921. The results are reported below under C, D and F'. 

 This corn was planted in two places in North Carolina, on soil quite 

 similar. Since the locations chosen were only 20 miles apart, the 

 climate was practically the same. The seed corn planted contained 

 9.07% protein. The sample grown in West Virginia showed 8.68%. 

 One sample grown in North Carolina contained 9.57%, the other, 

 9.62% protein. The two corns grown in North Carolina show very 

 nearly the same amounts of protein. Each had increased by 0.6^0 

 protein. We also made analyses of the White Plume corn, seed grown 

 in 1921 in North Carolina containing 8.41% protein. This corn was 

 planted in North Carolina and in West Virginia. The analyses are 

 given below under A' and B. The corn grown in North Carolina in 

 1922 contained only 7.75% of protein, a decrease of 0.66%. The 

 sample grown in West Virginia contained 8.37 %i, — scarcely any 

 change in protein. 



The yellow corn, Golden Dent, low protein corn, contained 6.82% 

 of protein. This seed was grown in North Carolina in 1921. The 

 sample grown in West Virginia in 1922 contains 8.22%, an increase 

 of 1.40% of protein. 



