252 KARL B. KRISTOFFERSON 



minated about 90 — 100 %, although they were stored during 

 winter. 



The results obtained are merely preliminary. However, the experi- 

 ments deal with species hybrids and some characters hitherto unknown 

 from a genetical point of view, and I believe this to be sufficient reason 

 for publishing the results. Furthermore, the pure lines obtained are 

 now lost, and the taking up of the experiments would therefore 

 require quite new material. Besides, I do not see any chance to start 

 the experiments again for the fourth time in the immediate future. 



MATERIAL AND METHODS. 



The following forms have been used in these investigations: 

 1 ) \ . tricolor L. subsp. gennina Wittr. f. versicolor Wittr. Line 10. 



2) V. tricolor L. subsp. coniophila Wittr. Line 41. 



3) V. tricolor L. subsp. ammotropha Wittr. Line 40. 



4) V. arvensis Murr, subsp. patens Wittr. f. scanica Wittr. Lines 

 3, 4 and 5. 



5) V. arvensis Murr, subsp. communis Wittr. Line 2, 13, 26 and 27. 



6) V. arvensis Murr, subsp. c«r//5epa/« Wittr. Line 25. 



Of these forms n:o 1 — 4 were quite identical with Wittrock's 

 (1897) forms. N.o 6 differed somewhat from Wittrock's subsp. cur- 

 tisepala; the end-lobe of the stipulae was a little shorter and the 

 pollen-magazine more closed than in Wittrock's forms. N:o 5 was an 

 intermediate form between V. arvensis communis and V. arvensis 

 patens scanica. It was most like the former subsp. in the vegetative 

 parts but the pollen-magazine was of the same appearance as the 

 latter one's. 



All these forms were collected in Skane. N:o 2, 3 and 6 along 

 the coast; the other in the inland. 



The methods afford nothing remarkable. The castration is easily 

 made. In V. arvensis it is necessary to castrate in an early stage as 

 the anthers burst already in the bud. In V. tricolor one may wait 

 till the day before the opening of the flower. The most handy method 

 of pollination is to loosen the spur-petal of the male parent care being 

 taken not to spill the pollen; it is then brought in contact with the 

 stigma of the plant to be crossed. The labellum of the stigma collects 

 the pollen, which then falls into the hollowpart of the stigma. Of 

 course, every possible precaution was taken; pollen was taken only 

 from isolated flowers, and scissors and pincers were carefully steri- 



