240 VENOMOUS SNAKES AND THE PHENOMENA OF THEIR VENOMS 
In a later work Noguchi ' prepared a pure crotalus antivenin with unmodi- 
fied venom and used it to determine the specificity of antivenin in regard to 
toxicity in vivo and hemolysis in vitro. ‘The results are as shown in table 24. 
TABLE 24. 


Antivenin of Species. Protection in vivo. paises eee 
Crotalus adamanteus: I c.c. neutralized — 
r2m.l.d.+2.5c.c. ....| Survived (no hemorrhage)... tom.h.d. 
Ancistrodon piscivorus: 
TaN Gl tae Cs Cr rre eeke 
ain 2 250 C.Gc etree 
Naja tripudians: 
TN laGs--2s5iCrG, sieves 
Daboia russellii 
Lachesis flavoviridis 

CROTALUS TERRIFICUS ANTIVENIN. 
Brazil? immunized mules and horses with the venom of Crotalus terrificus, 
or cascavel of Brazil. This antivenin neutralizes the toxic action of the 
same venom as well as that of Lachesis jararacussu, but not that of Lachesis 
lanceolatus, L. alternatus, and L. neuwiedit. 
ANCISTRODON PISCIVORUS ANTIVENIN. 
Noguchi * obtained pure antivenin for water-moccasin venom by treating 
goat with modified venom followed by injections of unmodified venom. As 
the supply of venom was insufficient he was unable to raise immunity to any 
high degree. The antivenin which he got was, however, sufficiently strong 
to determine the point in question. The results may be summarized in 
table 25. 
TABLE 25. 
Antihemolytic power 
Antivenin of Species. Protection in vivo. in vitro. 
Ancistrodon piscivorus: I c.c. neutralized — 
Gun id} 20 1C: Ca eier ct i 40 m.h.d 
Crotalus adamanteus: 
Bn VG? 2.5 C:Gs 1 oes i 4.25 
Aim dir 2i6 1 Cicyy ears - i 
Naja tripudians: 
mn yt 295 Cs Cs sereua sie 
Daboia russellii 
Lachesis flavoviridis 

LACHESIS LANCEOLATUS ANTIVENIN.* 
The antivenin prepared by Brazil by immunizing animals with the venom 
of Lachesis lanceolatus has proved to be very effective against this venom and 
also those of Lachesis alternatus and L. neuwiedii, but not at all so against 
1 Noguchi. Therapeutic experiments with anticrotalus and antimoccasin sera. Jour. of Exp. Med., 
1906, VIII, 614. 
2 Brazil. Contribution 4 |’ étude de l’intoxication d’origine ophidienne. Paris, 1905. 
3 Noguchi. Therapeutic experiments with anticrotalus and antimoccasin sera. Jour. of Exp. Med. 
1906, VIII, 614. 
4 Brazil. Loc. cit. 
