INTERACTIONS BETWEEN VENOM AND ANTIVENIN 251 
The rise of the minimal hzmolyzing doses through the fractions of the 
serum necessary for complete neutralization was as follows: 
TABLE 27. 
0.001 gm. Venom alone .. «<0... se ae 0.000005 gm. 
+0.1 C.c. Serum ...... 0.000025 
ea IS tie ai ataile 0.00005 
ROTA ee le et cede 0.00008 
PLO: Gta! bee ie We eace, bro) aise 0.00017 
oe ee er eras 0.0005 
In another way the above results can be summarized as follows: 
0.001 gm. of venom contains 10,000 + 5 = 2,000 minimal hemolyzing 
doses. With o.1 c.c. of serum necessary for a complete neutralization it now 
contains 10,000 + 25 = 400 minimal hemolyzing doses. If we arbitrarily 
call the number of combining equivalents in 0.001 gm. of venom 130, the above 
fraction neutralized 1,600 minimal hemolyzing doses per Io equivalents. On 
the theory of simple neutralization we should have neutralized 2,000 + 13 = 
153 minimal hemolyzing doses. Myers gives the following calculations: 
TABLE 28. 
Combining | M. h. d. removed 
equivalents. | by 1 equivalent. 
Venom alone 
+0.1 c.c, serum. . Io 160 
+0.2 aie Io 20 
+0.4 ae 20 3-75 
+0.6 a ; : 20 a3 
+0.8 oe : 20 1.9 

It will be seen at once that the interaction of the cobralysin and its antilysin 
is very different from that of a single neutralization, say of a strong acid by a 
strong base. If we explain this reaction according to the toxoid theory of 
Ehrlich, as was done by Myers, the results may be represented as in figure I. 

Varo Oe it. Ol Os | Ga unm Oe 09 hain Ree Ne 
- Fic. 2. 
