INTERACTIONS BETWEEN VENOM AND ANTIVENIN 255 
and Noguchi,’ who confirmed the results obtained by Myers with cobralysin 
and then extended this sort of observation to the neurotoxin of the same 
venom. ‘The cobra venom which they worked with contained 1,000 minimal 
hemolyzing doses in o.oo gm. for the test volume of defibrinated guinea-pig 
or dog’s blood (1 c.c. of 5 per cent suspension in 0.85 per cent NaCl). The 
antivenin was that of Calmette. First the fresh solution of venom was neu- 
tralized with antivenin. 0.002 gm. were completely neutralized by ae, 
of the serum. By partial neutralization the results shown in table 31 and 
figure 7 were obtained. 
They then subjected the venom solution to the influences of room tempera- 
ture and of 37°C. for a period of rg days, under aseptic conditions. These 
solutions showed very marked diminution in their hemolytic action. The 
neutralization with antivenin gave the results shown in table 32 and figure 8. 
TABLE 32. 





sponding frac 
of antivenin. 




Room-kept venom alone .. 
0.002 gM. +0.025 C.c. serum 
+0.05 et 
+0.075 
+0.1 
+0.125 

20 ¢ 
eH eH ee Db 
OFRwWNO AWD OO 
HHNU AN HHO O 
O 0.025 0.05 0.075 0.1 0.125 O15 0175 0.2 0.225 
Fic. 8. 
The neutralization of the venom solution kept at 37° C. for 19 days showed 
the results given in table 33 and figure 9. 
TABLE 33. 
k M. h. d. 
A neutralized 
8 a «| by corre- 
‘ sponding 
2 * | fraction of 
6 ~ 
=) ay 
+ 37° C. venom alone 
0.002 gm. +0.025 c.c. serum 
: +0.05 ae 
2 +0.075 
1 +0.1 
i 1 j +0.125 
© 0.025 0.05 0.075 01 0.125 0.15 

Fic. 9. 

1 Flexner and Noguchi. Constitution of snake venom and snake sera. Jour. of Path. and Bacteriol., 
1903, VIII, 279. 
