INTERACTIONS BETWEEN VENOM AND ANTIVENIN 259 
With another preparation of Calmette antivenin, much more powerful, and 
4 c.c. of which neutralized 0.003 gm. of the venom, the results shown in 
the third column of table 36 were obtained. 
Figure 12 shows the results of experiments with the first antivenin and 
Figure 13 those of the second antivenin. 
The results of partial neutralization of cobra hemolysin by the antivenin 
are as follows: For the test 8 c.c. of 1 per cent suspension of washed horse 
corpuscles (each liter containing 8 c.c. of o.or 7 lecithin) in each tube were 
used. ‘The test dose of venom was 1 c.c. of 0.1 per cent cobra venom. ‘Two 
preparations of antivenin were used (table 37 and fig. 14). 
TABLE 37. 





I C.c. 0.1 per cent cobra venom 
+ nc.c. of antivenin I 
+ 1-n c.c. of 0.9 per cent NaCl. 
I C.c. 0.1 per cent cobra venom 
n c.c. of antivenin IT 
+1-n c.c. of 0.9 per cent NaCl. 

100 n. q obs. q calc. 
= 
aR 
° 
> 
a 
eS 
B 
o 








30 o.1 83-5 82.5 
oO. 78.2 79-5 0.15 71.3 74 
80 0.2 56.6 59-5 0.2 64.5 66 
0.3 39-8 40.5 0.25 56.2 57 
- 21.6 0.3 44.4 48.5 
70 5-3 0.4 33-3 32 
0.5 15-9 15 
i 354 

60 
50 
40 
30 


0 0.1 0.2 0.3 0.4 0.5 0.6 
o Neutralization with antivenin I. 
p Neutralization with antivenin II. 
Fic. 14. — tela i antilysin. Horse blood with 
lecithin. 
WATER-MOCCASIN-VENOM ANTIVENIN. 
With this venom the partial neutralization with its specific antivenin gave 
a singular result. When 2 c.c. of the antivenin were added to 10 minimal 
lethal doses of the venom (0.012 gm.) the mixture contained about 6 minimal 
lethal doses, but with 8, 10, 20, and 40 c.c. there was no complete neutralization. 
Madsen and Noguchi left this phenomenon to a later investigation, not 
having enough material for further work at that time. 
The neutralization of the hemolytic component of water-moccasin venom 
with the specific antivenin gave the following results: 8 c.c. 5 per cent suspen- 
sion of dog blood in o.g per cent NaCl were used for the test reaction. 
