Efferent Neurones in the Electric Lobes. ; I 
OBSERVATIONS ON THE EFFERENT NEURONES 
IN tHE BLECTRIC: LOBES: OF TORPEDO :OCCI- 
DENTALIS. 
By SHINKISHI HaTal, 
(From the Biological Laboratory of the University of Cincinnati.) 
CONTENTS. 
I. Materials used and technique employed in the present 
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II. Finer structure of the efferent neurones of the electric lobes 
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III. Finer structure of the ground substance of the spinal gan- 
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IV. Remarks concerning the structure of the ground substance 
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I. MATERIALS USED AND TECHNIQUE EMPLOYED 
IN THE PRESENT INVESTIGATION. 
For the present investigation, the efferent neurones in the 
electric lobes of 7Zorpedo occidentalis, and the spinal ganglion 
cells from the mid-cervical ganglia of the adult white rat 
were used. The body weight of the rat was 141 grams. 
The torpedo material, which was generously furnished by 
Dr. Ayers, had been preserved with 10% formaline. To pre- 
pare this, a thin piece was cut from the lobe and transferred 
to distilled water for about six hours in order to remove all 
the formaline. After thorough washing with water, the 
material was transferred to 35% alcohol, where it remained 
about one hour, and then it was carried through graded 
alcohols and imbedded in paraffine in the usual way. The 
sections were cut 12 » in thickness. For staining, a satur- 
ated aqueous solution of toluidin blue, and for contrast 
staining, an alcohol solution of erythrosin, were used. 
Jour. Cin. Soc. Nat. Hist., VOL. XX, No. 1. I PRINTED OCTOBER I, Ig0I. 
