Opalina. sae 
plasm of Opalina is spoken of as containing intestinal contents. [It 
is, I think, always free from food particles or foecal matter, as STEN 
had already shown.| He confirms TOnnices’ statement that the re- 
fractive spherules contain granules, but finds no sign of alveolar 
structure in them. |My work confirms TOnxnices in the latter re- 
gard.| He saw no indications of division of the refractive spherules. 
The longitudinal striae, between the rows of cilia, he describes as 
compesed of rows of granules. {My study indicates that this always 
hazy appearance of granules (Fig. 2, Pl. XIV) may be an optical effect 
produced where the longitudinal ridges (Marer) of the pellicula pass 
above the transverse fibrillae (TénNicEs) of the subpellicular net- 
work which is connected with the basal granules of the cilia.| 
LéwentHat (1904) describes for O. ranarum the formation of 
the chromatin spheres in the nuclei before encystment (Text Fig. X, 
page 280). He distinguishes the more strongly staining sphere from 
the less deeply staining [and, according to my own observations, gra- 
nular| mass, saying that the former arises from the latter. The 
deeply staining compact sphere he regards as homologous to a micro- 
nucleus (sexual), the weakly staining residue to a macronucleus (nu- 
tritive). His figures show what he believes to be the sequence of 
phenomena. |The darkly staining spheres are extruded from the 
nucleus, as NERESHEIMER and I have shown, and go to pieces in the 
cytoplasm. They are probably composed of nutritive chromatin.} 
Conn (1904) gives an account of O. intestinalis, which is either 
inaccurate in most points, or is based wholly or in part on abnormal 
animals or on some other form or forms. Some of the features 
described [which do not fit normal O. intestinalis] are: that the body 
is often triangular and flattened; that the refractive spherules 
disappear after twenty-four hours if the animals be kept without 
food in a hanging drop in a moist chamber; that the alveoles of the 
cytoplasmic foam grow smaller from the center of the body toward 
the periphery; that small forms are never binucleated and large 
forms never uninucleated. The individuals figured in conjugation 
are evidently not Opalinas. The budding described appears, to have 
been pseudoencystment following fragmentation. 
Licer & Dusoscg (1904a@ and ) give a fine account of the 
structure of an interesting new species, O. saturnalis, which is found 
in the rectum of Box boops, a fish from the Mediterranean Sea. ‘This 
is the only Opalina which is reported from a host which is not an 
Amphibian. The authors describe elongated and stocky forms {as 
in O. caudata|; “lecithin (?)” bodies [my “ectosare spherules”| are 
