Opalina. 345 
chromatin. In “Paramaecia” [probably Balantidiwm] from the rectum 
of the frog, and in other Cvtliata, Girmsa’s staining colors the 
micronucleus red, the macronucleus blue. [The finer nuclear granules 
in Opalina I have regarded as chiefly achromatic, being influenced 
largely be their behaviour in degenerating nuclei of 0. obtrigona, 
and by the fact that after staining with safranin and light green 
they are green, not red.| 
