370 M. M. Metcaur 
Fig. 209. A living animal from a tadpole of Bombinator pachypus, infected 
88 hours. The nuclei were not perfectly clear. In the original free-hand sketch 
they are annotated with a question mark. 
Plate XXV. 
Opalina intestinalis. 
All figures are reduced one-third, to the magnification indicated. 
Figs. 210—218. Successive stages of copulation and pseudoencystment of a 
pair of gametes from a tadpole of Bufo vulgaris. Fig. 210 is a free-hand sketch 
from memory made immediately after the male become attached; the other figures 
< 673 diameters. From life. 
Figs. 219—221. Pseudocysts from tadpoles of Bombinator pachypus (Figs. 
219 and 221) and Bufo vulgaris (Fig. 220). In Fig. 219 four granular chromo- 
somes are seen in each end of the dividing nucleus. The granules apparently 
were not arranged in pairs, their number in two of the chromosomes being uneven. 
In Fig. 220 each macrogamete nucleus had four chromosomes the uppermost two 
of which in each case showed distinct granules, so arranged however as not to be 
readily counted; the granules of the lower two chromosomes were less distinctly 
seen. The nucleus from the microgamete showed a single compact chromatin 
mass which contained a group of granules at one side. The excretory vacuole 
with faintly visible granules (in Browntan movement) is shown near the top of 
the figure. Fig. 219 from life, & 990 diameters; Fig. 22U from life, X 673 dia- 
meters; Fig. 221 acetic-carmine, * 673 diameters. 
Figs. 222—225. Zygotes with peculiar spindle-shaped syncaria, from tadpoles 
of Bombinator pachypus, infected respectively 7'/, days, 88 hours, 7 days, 113 hours. 
In Fig. 224 a vacuole of the excretory organ is shown in dotted outline. In Fig. 
225 the large disc in the nucleus was probably not a nucleolus but a mass of 
chromatin. Figs. 222 and 224 acetic acid; Fig. 223 acetic-carmine; Fig. 225 from 
life. >< 673 diameters. 
Figs. 226 and 227. Zygotes whose daughter nuclei somewhat resemble the 
nuclei shown in Figs. 222 and 224; from a tadpole of Bombinator pachypus, in- 
fected 7'/. days. Acetic acid. > 673 diameters. 
Figs. 228—235. Abnormal individuals from tadpoles of Bombinator pachypus, 
infected 43 hours (Fig. 228), 54 hours (Figs. 229—233), 91 hours (Fig. 234), and 
114 hours (Fig. 235). These tadpoles were infected from two different lots of 
cysts, Figs. 229—233 being from one series of infections, Figs. 228, 234 and 235 
from another. From life. Figs. 284 and 235 free-hand sketches; the rest camera 
drawings < 673 diameters. 
Fig. 236. A section of an infection cyst from a tadpole of Bombinator pachypus. 
Coros. subl.-acetic acid, Detarrenp’s haematoxylin. No record was made of the 
infection or of the magnification of the drawing. 
Plate XXVi 
Opalina intestinalis and O. caudata. 
All figures are reduced one-third, to the magnification indicated. 
Figs. 237—247. Opalina intestinalis. 
Figs. 237—239. Three sections of a small individual which passed unencysted 
through the alimentary caual into the rectum of a tadpole of Bombinutor pachypus. 
