Opalina. 375 
nuclei irregularly arranged instead of in an axial row, were present in the same 
tadpoles. Acetic-carmine. > 673 diameters. 
Figs. 325—327. Opalina ranarum. 
Fig. 325. A minute individual ready for encystment, from the rectum of an 
adult Rana temporaria. The endosare spherules (unshaded) are shown; cilia are 
not drawn because injured. The method of preparation was not noted, but the 
injury to the cilia makes it probable that acetic-carmine was used. >< 673 diameters. 
Fig. 326. A cyst from the same host. Cilia were present within the cyst 
but were too confused to draw. The method of preparation was not noted. >< 673 
diameters. 
Fig. 327. A section of a zygote in the rectum of a tadpole of Rana tempo- 
raria. The nucleus is carefully drawn, as are also the endosarc spherules. Coros. 
subl.-acetic acid, iron haematoxylin. > 990 diameters. 
