FLOWER AND SEED COLOUR IN LUPINUS 309 
122 blue: 40 bluish red (table 10) 
expected: 121,5 : 40,5 D/M = 0,5/5,51 = 0,09. 
The following segregation numbers were obtained in F;: 
296 blue: 101 bluish red 
expected: 297,75 : 99,25 D/M = 1,75/8,63 — 0,20. 
The relation between the number of constant and segregating fa- 
milies is 6: 8, expected: 4,67: 9,3. The constant blue families have 
296 individuals in all. From 6 bluish red parent plants 202 indivi- 
duals of the same colour were obtained. Thus the results are pretty 
well in accordance with the theoretical expectation. 
Cross 4 (tables 12—13). 
Tinged blue X blue flower colour, no difference in seed colour. 
RRBBVVff RRBBVVFF 
F,, having the formula RRBBVVFf and heterozygotic in factor F 
only, is blue, but upon closer study it is found that a weakening of 
the colour has taken place. The influence of the F-factor is thus 
seen to be somewhat stronger in the duplex stage than in the simplex 
stage. The segregation numbers in F, as well as in F, are very good: 
F, 396 blue : 138 tinged blue (table 12) 
expected: 400,5 :133,5 D/M = 4,5/10,0 = 0,45 
F; 324 blue : 107 tinged blue (table 13) 
expected: 323,25 : 107,75 D/M = 0,75/8,99 = 0,0. 
The relation between the constant and the segregating F;-families, 
6 : 11, comes in addition as close as possible to the theoretical expecta- 
tion, and the progenies of all tinged blue coloured plants investigated 
have been found to be constant in accordance with the theory. 
Simple Mendelian segregation is to be expected in some other 
combinations according to the theory. No primary crosses have been 
made in these cases, but the F; of some di-hybrid crosses ineludes 
families from parent plants with the same kind of heterozygosis as 
that of the primary crosses if made. The proportions to be expected, 
3:1, are found in all cases. The following cases should be observed: 
Tinged blue X tinged red in F, of cross 10 (table 26), 
Bluish red .X pure red in F, of cross 9 (tables 23—24), 
Violet X pure red in F; of cross 9 (table 24), 
Bluish red > tinged red in F, of cross 10 (table 26), 
