74 Journal of Entomology and Zoology 



paraffin of a melting point of 60-62 degrees, but it was necessary 

 to peel off the cuticle and withdraw the setae of those fixed in hot 

 mercuric chlorid. The internal connective tissues, however, were 

 better preserved in this fluid. There seemed to be no marked dif- 

 ference in fixation of the other tissues. 



Staining. The most effecti\e stain for tracing out gross anatomy 

 and nerve distribution proved to be a faintly acidified borax car- 

 mine used after a fixation of hot mercuric chlorid. The muscular 

 and epithelial tissues were stained a uniform light pink, the nervous 

 tissues a darker pink and the connective tissues, especially the peri- 

 neurium a bright red. Villain's copper hematoxylin, iron hema- 

 toxylin, and double stains of these and methylin blue with eosin, 

 "licht griin" and neutral red were of value for cellular detail. 



Reconstruction Method. A wax and blotting paper model of the 

 brain (Fig. 2), was made in the usual way. To supplement this 

 two stereographic reconstructions (Figs. 1, 4) were made which 

 were of advantage over the wax model in the following ways. They 

 are easier to make, eliminating the steps that involve cutting out, 

 impregnating and reassembling the parts of the wax reconstruc- 

 tion. It is possible as was done in Fig. 1 to make a "sciagraphic 

 reconstruction" that will show the internal parts in their normal 

 relation to the other organs. If necessary, colors could be used 

 to make the morphological differentiation clearer. When the re- 

 construction is finished it is the reproduction at a certain magnifica- 

 tion of any desired aspect of the object and is equal in all import- 

 ant respects and superior in many respects to a photograph or draw- 

 ing of a wax reconstruction. What little distortion there is may 

 be calculated as a function of the displacement angle and easily 

 taken into account. 



The method pursued may be described as follows: The object 

 should be embedded with a piece of liver or similar tissue having a 

 smooth and quite rectangular face parallel to the axis of the speci- 

 men. The pieces may be arranged on a watch crystal after infiltra- 

 tion and the embedding paraffin poured on hot enough not to form 

 a crystallization capsule around them. This gives an orientation 

 guide whose projection is represented in b (Pig. 14). Pig. 14 is a 



