32 .STORRS AGRICULTURAL EXPERIMENT STATION. 



7. The number of liquefiers was excessiv^ely high, there 

 being at the outset about 30 per cent, of the bacteria that 

 hquef}' gelatin. This number remained quite high throughout 

 the whole series. There was noticed an increase in percentage 

 at the end of six hours, but that was followed by a somewhat 

 rapid decline and at twenty-four hours the proportion had 

 dropped to 7.5 per cent. There was an increase in total num- 

 bers, however, up to the very last test. 



8. The miscellaneous bacteria, of which three species are re- 

 corded, were not abundant, the numbers being only a few hun- 

 dred and the proportions less than 4 per cent. It will be seen, ' 

 moreover, that while there was slight increase in actual numbers 

 of these bacteria for a few hours, there was a constant decline 

 in percentage, and in the tests that were made at twenty-four 

 hours these species did not appear at all. This does not neces- 

 sarily indicate that they were totally absent, because the dilu- 

 tions that were necessar}^ at twenty-four hours were so much 

 greater than those required at the end of six hours that a small 

 number of these miscellaneous species would be very likely to 

 escape observation in the plates. While, therefore, their ab- 

 sence from the tables in the figures for the later tests indicates 

 that they had not increased in number appreciably during the 

 experiment, it does not indicate that they were totally absent 

 from the milk. 



9. The number of colonies that were undetermined in these 

 tests is shown by the last column to be somewhat variable, in 

 one test rising to 12 per cent., but in the other cases being 

 about 3 per cent, or less. 



Experiment No. 2. November. — This experiment was a rep- 

 etition of the last and occurred five days sub.sequently. The 

 conditions of the experiment were identical with those of Ex- 

 periment No. I. The results of the tests made up to the age 

 of twenty-four hours are shown in the two following tables. . 



