EK. W. BERGER ON THE CUBOMEDUSA. 37 
minutes more only three. There was a long latent period—two or 
three seconds—before the stimulation of cutting off the arm made 
itself evident in the rhythm. 
A second oral lobe was removed. Then there followed twenty-four 
pulsations, a pause of two seconds, and two pulsations, in all twenty- 
six pulsations to a minute. The rate of pulsation soon fell to the 
previously abnormal low rate. 
Third lobe removed: 21 pulsations in first half minute and then 
16, or 37 per minute. 
Fourth lobe removed: 17 pulsations in first half-minute plus 13 
gives 30 for the minute. 
No difference in the coordination of the animal was shown as a 
result of the removal of one-half the number of oral arms. 
Fifth lobe removed: 17 pulsations plus 15 equals 32 to the minute. 
Sixth lobe removed: 17 in first half-minute plus 4 in the second 
half-minute gives 21 pulsations for the minute. 
Seventh lobe removed: 17 plus 9, or 26 per minute. 
In all these instances the rhythm in the second half of the first 
minute was irregular and intermittent. 
Seventeen and then seven pulsations were provoked after the 
animal had become quiescent, or nearly so, by merely handling it. 
45. Eighth oral lobe was removed and pulsations stopped. The next 
day the animal was in good condition. The pulsations counted in the 
evening were 12, 14, 14, 11, per minute. The rhythm was not 
regular; there was a tendency to groups of twos, threes, or more, but 
no prolonged intervals of rest were observed. When placed into 
fresh sea-water, the pulsations were fourteen to the half-minute or 
twenty-six to the minute; seventeen to the half-minute, and thirty- 
three to the minute were also counted. This specimen gave spon- 
taneous contractions during two weeks, after which it was thrown 
out, the aboral end being eaten through and little or no regeneration 
having taken place. 
46. Two more were operated upon: A. Its rhythm was 18, 14, 17. 
Its entire margin was cut off. The separate pieces of the margin 
pulsated, 6, 7, 4, 6, 7, 9. The animal seemed paralyzed by the 
operation; it responded by a contraction now and then to stimu- 
lation but gave no spontaneous pulsations. B. Its rhythm was 17, 
15, 12, 12. All its oral arms were removed. Its rhythm was only 
raised to seventeen and not perfect. In twenty-five minutes it had 
fallen to eleven, in four hours to ten pulsations [per minute]. 
