38 JOHNS HOPKINS UNIVERSITY MORPHOLOGICAL MONOGRAPHS. 
May 22nd. A and B are living as also the pieces of the margin 
of A; all are giving spontaneous pulsations now and then at compar- 
atively long intervals—even A, with its margin removed. 
May 26th. Everything is still living. The one with the margin 
cut (A) counted sixteen and nineteen pulsations per minute, though 
this was not kept up all the time. 
June 2nd. A and B and pieces are still living and contracting 
spontaneously. It is now two weeks, and they were thrown out eaten 
through at the aboral end with little or no regeneration. 
47. The margin was cut off another one (C) and it was then 
paralyzed. The margin contracted vigorously by itself. The margin 
was next split, but a connection of about one-half an inch wide was left 
between the two rings. Over this bridge the contractions passed 
from the outer and inner ring. The inner ring did not originate 
any contractions. Both rings were then cut near their connecting 
bridge of tissue and the larger ring with the marginal bodies was 
split longitudinally so as to separate the exumbral from the sub- 
umbral portion. It was found that the contractions started only from 
the subumbral portion while the exumbral portion did not contract 
at all. 
June 5th. Five of the eight small pieces of C were not seen to 
contract either to-day or yesterday. A slow rotary motion was 
observed in some of the pieces suggesting ciliation, but no cilia or 
currents pointing to ciliation were seen with a low power. C was 
seen to pulsate spontaneously. Possibly it did yesterday but it was 
not watched closely. A piece of the subumbral surface of C broken 
off (not from the margin) was found to contract spontaneously. 
48. June 6th. In a fresh one (D) from Port Royal, the eight litho- 
cysts of one side were removed in order to compare its movements 
with an intact one. Codrdination was apparently unaffected. 
June 9th. The margin of C is still pulsating vigorously. Parts 
of the subumbrella broken loose from the strip pulsated by them- 
selves now and then. Fifteen lithocysts were removed, leaving only 
one at the end of the strip. It was found that with this single 
ganglion (lithocyst) left, and originating most of the contractions, now 
and then a contraction would originate at another part of the strip 
where there was no ganglion. Three days later contractions 
originated as often from other parts as from the ganglion. 
