34 PROLIFERATION IN CONTROL OF BOLL WEEVIL. 



It should be statcMl that the attempt to seal punctures l)y applyino; a 

 solution of a^ar-afijar was not successful, as upon drymj^: it would peel 

 away from the boll, leaving the puncture practically open. The 

 sealing with a solution of shellac resulted successfully in most cases. 



Among the 1,103 artificial punctures made proliferation resulted 

 in 36.8 per cent. While this percentage is hardly half that found in 

 Table II for feeding punctures of the weevil, it seems fidly as large 

 as should be expected from the unfavorable conditions prevailing in 

 these tests. Among the 604 unsealed punctures at Dallas prolifera- 

 tion resulted in 30 per cent. Among the 223 instances of proliferation 

 recorded from these unsealed punctures 62.8 per cent were from 

 the inner side of the carpel, 36.3 per cent were in the seeds, and 1 per 

 cent in the septa separating the locks. A comparison with the 

 results from the 38 sealed punctures at Dallas shows in the latter case 

 proliferation formed in 44.7 per cent of the punctures. Among the 20 

 instances of proliferation resulting, 85 per cent occurred in the carpel 

 and 15 per cent in the septa. 



COMPARISON OF RESULTS FROM SIMPLE NEEDLE PUNCTURES WITH 

 EFFECTS OF CHEMICAL INJECTIONS. 



Comparing next the results from simple needle punctures with 

 those from chemical injections for the Dallas experiments, it is found 

 that from the needle punctures proliferation resulted in 24.1 per cent 

 of the total cases, while from the chemical injections it resulted in 45.9 

 per cent of cases. It should be stated, however, that decay was much 

 more common in the cases of chemical treatment, and in many locks it 

 was impossible to tell whether the decay had closely followed or 

 whether it had caused the proliferation. 



An examination of the records for Hidalgo shows that proliferation 

 resulted from 44.6 per cent of all simple needle punctures and from 

 45.1 per cent of those receiving chemical injections. 



COMPARISON OF RESULTS FROM SEALED AND UNSEALED PUNCTURES. 



In a comparison of results from 164 sealed and 630 unsealed simple 

 needle punctures it is found that proliferation resulted in 53.7 percent 

 of the sealed punctures and in 28.1 per cent of those unsealed. In the 

 chemically treated punctures proliferation resulted in 47 per cent of 

 the 249 unsealed and in only 40 per cent of the 60 sealed. As these per- 

 centages in chemically treated punctures stand in inverse proportion 

 to those in simple needle punctures, it does not appear that a well- 

 founded conclusion can be drawn as to the influence which the sealing 

 of punctures may have upon the subsequent formation of proliferation. 



