New York Agricultural Experiment StatiOxN. 409 



widely over tlie surface of the agar. In streak cultures there is 

 <:onsiderable growth at the end of twenty-four hours at the room 

 temperature. This growth is at first dirty yellowish-white, but 

 gradually changes to a deep yellow (a color expert pronounced it 

 '^ raw sienna "). The margins of the growth are quite strongly 

 lobulated, but well defined. The surface is at first smooth and 

 shining, but as the water evaporates from the agar granulation 

 takes place, commencing in the upper part of the tube. It does 

 not grow down into the agar nor color the agar. No odor is pro- 

 ■duced. In stab cultures there is considerable growth along the 

 needle-track. The " nail-head " growth is thick, its margin is 

 slightly irregular and of a deeper yellow than the interior. For 

 a considerable length of time the surface is smooth but eventually 

 it becomes granular as in streak cultures. 



The organism grows readily, also, on potato agar which is 

 prepared by adding ten grams of agar to one liter of potato broth. 

 The color and habit of growth are practically the same as on beef 

 agar. 



Q-roicth on gelatin. — The gelatin used was the following com- 

 composition: Beef, 500 grams; Witte's peptonum siccum, 10 

 grams; gelatin (Compte Fils k Madgebourg, first quality), 150 

 grams; water^ 1 liter; neutralized with sodium carbonate. On 

 this medium, at the same temperature, the organism grows less 

 rapidly than on agar. In streak cultures the growth is narrow, 

 quite thick, smooth and shining on the surface and smooth along 

 the margins. The color is a trifle lighter than on agar, and there 

 is no granulation until the seventh or eighth week. There is no 

 growth down into the agar. In stab culture the " nail-head " 

 growth is of small diameter, thick, smooth and shining, smooth 

 around the margin and of orange-yellow color. Along the needle- 

 track there is considerable of a dirty yellowish-white growth. 

 The needle-track does not become funnel-shaped. There is no 

 liquefaction of the gelatin. 



Growth on potato. — On sterilized slanted potato cylinders the 

 organism grows very rapidly. Potato seems to be its favorite 

 culture medium. At a temperature of 2G°C. there is a copious 



