410 Report of the Mycologist of the 



growtli in twenty-four lioiirs. It spreads irregularly over the 

 slanted surface, is smooth, and for three or four days has the 

 same color as on agar, but at the end of a week it is slightly 

 iridescent. In 48 hours the potato cylinder begins to turn brown 

 and in a week it is decidedly brown. A yellowish-white precipi- 

 tate forms in the water at the bottom of the tube. No gas bub- 

 bles are formed. 



GrQwtJi in houillon. — It grows in bouillon, either neutral or 

 made quite strongly acid with malic acid, but the growth is not 

 conspicuous. In bouillon containing 500 grams beef and ten 

 grams Witte's peptonum siccum per liter it produces, in twenty- 

 four hours, a slight cloudiness which increases very slowly. At 

 the end of three weeks some flocculent white precipitate is 

 formed, the liquid is moderately cloudy, and, if the tube has 

 remained quiet for several days, the surface will be covered by 

 a thin film bearing raised, yellow colonies of the size of a pin- 

 head. 



Growth in peptone solution. — The peptone solution of Dunham 

 was used. The formula is as follows: Distilled water, 1 liter; 

 peptone (Witte's peptonum siccum in the present case), ten grams; 

 sodium chloride, 5 grams. In twenty-four hours there is a slight 

 turbidity which becomes quite pronounced at the end of a week. 

 There is a small quantity of dirty- white precipitate in the bot- 

 tom of the tube, but no membrane forms on the surface. With 

 age, the precipitate becomes light yellow in color. 



Growth in milk. — Sterilized skimmed milk is changed very 

 slowly by the germ. By the fourth week there is usually a thin 

 layer (5 to 8 millimeters thick) of clear liquid in the upper part 

 of the tube, while the remainder appears unaltered. 



Chemical reactions produced hy its growth. — The pale rose color 

 of peptone-rosolic-acid solution is slightly intensified, indicating 

 the presence of a small amount of some alkali, but if this is the 

 case the quantity produced is not sufficient to overcome the 

 slight acidity of the water in the bottoms of tubes containing 

 sterilized potato cylinders. And the behavior of tubes of litmua 

 milk inoculated with the germ indicate the production of acid 

 rather than alkali. During the first three weeks the color of 



