SI 
308. BACILLUS TETANI b  —grows 
only in an atmosphere deprived of oxygen ; chemical 
absorption of the oxygen by pyrogallol, Buchner’s 
method 
(cause of tetanus or “lock-jaw’’). 
300. ze CEDEMATIS MALIGNI a—grows only in 
an atmosphere deprived of oxygen; mechanical 
exclusion of air by a layer of oil 
(cause of malignant cedema). 
310. 6s DIPHTHERL® a b c 
(cause of diphtheria). 
311. DIPHTHERIA TOXIN, resulting from the growth of the B. 
diphtheriz in broth. Used to inoculate horses and other ani- 
mals to induce artificial immunity. 
312. DIPHTHERIA ANTITOXIN; serum from the blood of a horse, 
rendered highly immune by repeated inoculations with the diph- 
theria toxins. Used hypodermically in the treatment of diph- 
theria as a curative agent, and also as a protective agent to 
those who have been exposed to infection. 
313. DIFFERENTIAL CULTURES OF TWO BACILLI, one patho- 
genic, the other not pathogenic under normal conditions, which 
are frequently associated and which in many respects resemble 
one another so closely that they may be confounded. 
Bacillus typhosus Bacterium coli commune 
(cause of typhoid fever) (abundant in the large 
intestine) 
Gelatin inconstant differences 
Agar do do 
Broth usually no pellicle formed. usually pellicle is formed 
Lactose—litmus 
agar turnsblue,ornochangeincolor change in color, turns red 
Fermentation 
test no gas formed gas is formed 
Potato spreading, invisible growth abundant yellow growth 
Milk not coagulated is coagulated 
Nitrates not reduced are reduced 
Indol not formed is formed 
314. CHART, showing the determinations used for identification 
of bacteria. 
All the preparations in this department are exhibited by Dr. 
T. M. Cheesman. 
