368 Wulist: BRANCHED PROTHALLIA 
Knop’s Solution Prantl’s Solution 
(a) Ca(NOs)2 omitted (a) NH«NOs omitted 
(b) NaCl and NasPOs omitted 
(c) NaCl omitted 
(d) NasPO, omitted 
(e) CaSO, omitted 
After the spores were sown, the cultures were placed before 
an east window where the conditions of light and temperature 
were approximately the same for all. 
DESCRIPTION OF THE BRANCHED PROTHALLIA 
In all the cases observed, the germination of the spores, the 
manner and the rate of growth in the branching prothallia did 
not differ from that of the unbranched prothallia. Neither were 
the branched prothallia limited to one kind of culture, but were 
found in all three kinds. In some of the genera they appeared 
simultaneosluiyn the three kinds of cultures. 
Fic. 3. Branched prothallia of Onoclea sensibilis L., X 75- 
Onoclea sensibilis L.—The prothallia of this species both on the 
distilled water and the soil cultures showed a decided ‘tendency to 
branch. Likewise, the branches showed a great diversity of 
origin and form, and the beginnings of apical cells or more advanced 
