508 SAX: FERTILIZATION IN FRITILLARIA PUDICA 
which shows them to be distinct cells. Their cytoplasmic con- 
tents are less dense and stain lighter than those of the ‘‘ Endo- 
pitisaasioced and antipodal cells. At each end of the ‘‘Endo- 
spermanlage”’ is a distinct membrane. At. the 
micropylar end the ‘‘ Endospermanlage ’’ may 
project up between or around the cells of the 
egg apparatus. There is a great deal of varia- 
tion in this respect, the ‘‘Endospermanlage’”’ ex- 
tending up around the cells of the egg apparatus 
at different points to varying distances. The 
upper polar nucleus is smaller and less regu- 
lar in outline than the lower one. It is always 
located near the periphery of the sac and sur- 
rounded by less cytoplasm. The central portion 
of the “ Endospermanlage ”’ consists of a large 
vacuole and a thin peripheral layer of cytop- 
lasm. The thickness of this layer is consider- 
ably less than the upper polar nucleus (TEXT 
FIG. I). 
Only one case was observed where the tube 
had reached the embryo sac but had not pene- 
trated it. The tip of the tube was enlarged and 
rounded and had turned along the nucellus 
towards one of the nuclei of egg apparatus, 
probably that of a synergid. In practically all 
: cases one of the synergids is destroyed by the 
entering pollen tube; but just what the relation- 
b * ship between the synergid and the pollen tube is 
two polar nuclei, the 
r the 
normal antipodal cell 
and one which is dis- 
organizing. 
could not be determined. In one case the cell 
as well as the nucleus in it, through which the 
pollen tube is about to enter, has a pointed end 
towards the end of the pollen tube. But two, 
or even all of the cells and nuclei of the egg 
apparatus, may be more or less pointed towards 
the micropyle. When the pollen tube is in con- 
tact with the embryo sac, the cells of the egg apparatus are all in- 
tact and show no signs of having emptied their contents into the 
micropyle, or even of having penetrated the wall of the embryo 
sac. 
