514 SAx: FERTILIZATION IN FRITILLARIA PUDICA 
one side of the upper polar nucleus. Several cases were observed 
where the male nucleus had both ends in contact with the upper 
polar nucleus (Fic. 29). Sometimes the male nucleus appears to 
have come in contact with the upper polar nucleus while still 
coiled. The male nucleus soon shortens 
and thickens along one side of the polar 
nucleus (Fic. 28). The contents of the 
male nucleus are denser and in larger 
pieces. The polar nucleus is not indented 
as is often the case of the egg, but often 
becomes flattened at the point of contact. 
The contraction and loss of the vermi- 
form shape of the male nucleus usually 
‘occurs before the two nuclei migrate to the 
lower polar nucleus. The male nucleus 
may, however, retain its vermiform shape 
during the time of its migration with the 
upper polar nucleus, or even after the two 
nuclei have reached the lower polar nu- 
cleus. TEXT FIG. 2 shows a longitudinal 
section of the entire embryo sac with the 
egg and one of the male nuclei in contact, a 
synergid, the upper polar nucleus and the 
second male nucleus in contact, beginning 
their migration towards the lower polar nu- 
cleus, and the antipodal nuclei. The male 
Fic. 2. Embryo sac nucleus in contact with the upper polar nu- 
uae aks cleus has lost its vermiform shape. The 
nucleus; the other male Cytoplasm of the upper part of the “Endo- 
nucleus and upper polar spermanlage”’ is massed mostly along one 
ites od aL ae side of the sac and especially around the 
polat thislens. two migrating nuclei. The strands of 
cytoplasm and the massing of the contents 
largely along the one side of the “ Endospermanlage” at the upper 
end is very characteristic. 
Fic. 31 shows the migrating nuclei almost in contact with 
the lower polar nucleus. The male nucleus is on the side nearest 
the lower polar nucleus and has retained its net-like structure. 
