562 KUNKEL: ORANGE RUSTS OF RUBUS 
nuclei lie close together near the end of the tube as is shown in 
Fic. 3. Sometimes they are separated by a distance of two or 
three hundred microns but they are never very far apart. The 
close proximity of the nuclei suggests that these germ tubes would 
give rise to sporophytic mycelium. The bases of the tubes appear 
quite empty while the protoplasm shows more and more density 
as we pass toward their apices. This is well shown in FIG. 3. 
The nuclei are strikingly large. They often show almost as great 
a diameter as the tubes that contain them. In some cases they 
have the appearance of ordinary resting nuclei with a distinct 
GYMNOCONIA INTERSTITIALIS (Schlect.) Lagerh. 
Fics. 1 and 2 show the long unbranched aecidiospore germ tubes as they appeaf 
in the living condition, X 175. Fic. 3 shows a germ tube stained; the two nuclei 
lie near the end of the tube where the protoplasm is dense, X 175. Fic. 4 shows 
the nuclei in the resting condition; nuclear membranes and chromatin strands are 
clearly seen, X 590. Fic. 5 shows the deep-staining nuclei; nuclear membranes 
are not to be observed and chromatin strands are indistinct, 590. 
nuclear membrane and blue staining chromatin strands. Such 
nuclei are illustrated in Fic. 4. In other cases, however, it is 
difficult to make out a nuclear membrane; the chromatin strands 
are quite dense and stain so deeply that the whole nucleus appears 
a dark bluecolor. Such nuclei are illustrated in Fic. 5. Innocase 
have nucleoli been observed and nuclear division figures seem to 
be absent from the preparations. One tube was found that had 
three nuclei, but this is a very rare case. The length of the germ 
tubes, the presence of only two nuclei, the large size of the nuclel, 
the absence of division figures and the lack of septa are all char- 
