Brooks : The Fruit Spot of apples 441 





Other inoculation experiments were made by tying sheets of 

 rubber closely around Baldwin apples and filling the bags thus 

 formed with water that contained an abundance of spores. Check 

 apples were similarly treated with water without the addition of 

 the spores. These sacks were left on the apples three days, and 



then removed late in the afternoon. 



J 



increased the number of spots by more than a hundred per cent, 



and those made in August showed a very definite increase over 

 the checks. 



One would seem to be justified in concluding from the sets of data 



above that the possibility of infecting the apples with the fungus in 



question decreases from July 5 to the close of the season and that 



the month of July is the time when the majority of the infections 

 naturally occur. 



On August 30, September 7, and September 12, 1907, attempts 

 were made to inoculate Baldwins and Northern Spies by introducing 

 the spores under the skin of the apple by means of a sterile plati- 

 num needle. Six inoculations and six punctures were made on 

 each apple. The apples were left on the tree till gathering time, 

 October 10. A hard corky growth developed around the needle 

 path in both punctures and inoculations. No difference could be 

 seen ln tne two at the time of gathering and no change in either 

 vas apparent in storage. Tissue from the inoculations when trans- 

 erred to culture media either gave no growth or a growth of 



eria. The fungus was evidently unable to develop in the 

 column of cider that must have filled the needle path after inocu- 

 a ion. T n j s f act is - m a g reement w j tn data given later showing 



a the fungus did not develop on Baldwin cider made from apples 

 gathered the last of August, even when this was diluted to one 

 thlr d its original strength. 



Characteristics of the fungus 



n order to learn as much as possible of the nature and iden- 



the fungus it has been grown upon a large number of cul- 



rnedia. Except where otherwise stated the decoctions used 



prepared in the manner prescribed in bacteriological and 



Pathological texts. 



n a 'l liquid cultures, except with a few very unfavorable 



