444 Brooks: The Fruit Spot of apples 



sugar the fungous growth was white or pink, but where sugar was 

 present as the principal ingredient it was olive or black. The 

 solution given below was found to give a very satisfactory growth 

 of the fungus and was used for all stock cultures. For the sake 

 of convenience it will be referred to hereafter as solution A. 



Glucose or sucrose 1° grams 



Apple bouillon 



25 c.c 



Sodium chloride » g ram 



Liebig beef-extract * g ram 



Peptone 2 grams 



Water 875 c.c. 



The following notes give some of the characteristics of the 



fungus in various culture media. 



In water. — Blocks of infected apple tissue two or three milli- 

 meters in diameter gave a good growth of the fungus when drop- 

 ped into sterile tap or distilled water. In less than a week the 

 fungus had fastened the blocks to the bottom of the test tube and 

 later spores were produced in limited numbers. The hyphae were 

 about 2 fi in diameter, hyaline, and sparingly septate. Spores 

 germinated readily in distilled water, producing hyphae severa 

 hundred jx long in a few days. 



In peptone beef bouillon. — In this medium the growth was a 

 pinkish white, beginning as colonies on the walls of the flask an 

 later forming a shiny pink layer on the surface of the liq" 1 ■ 

 There was a fair production of spores. The hyphae were coarser 

 and shorter than those described above. , , 



In glucose bouillon. — Olive colonies developed on the si e 

 the test-tube, followed by a dark olive growth on the surface. 

 Spores were produced in abundance and were always nyai • 

 The hyphae were thicker-walled and more abundantly sepa 

 than in other media. j 



In solution A (see above). — The growth was more rapid an 

 the spores were produced in greater abundance than in b 

 bouillon. The hyphae were hyaline except in the surface gr 



owt» 



of the 



In milk. — The fungus developed only on the surface ^ 

 milk. After seven days a slimy pink band of fungous gro^ 

 would be seen on the walls of the test-tube and a layer o 



