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reverted to its former condition. As trays No. 1 and 3, placed 
under the same conditions, showed no alteration in the daily 
average of deaths, this may be accepted as an instance of the 
effects of slight shocks in the earlier days of embryonic life, 
Thirteen days subsequently it became necessary to similarly 
change the eggs in tray No. 1, also containing about 2,500 
eggs, but the deaths were not increased thereby, as merely 5 
dead ones were picked out during the succeeding 14 days; 
whereas in the previous 37 days 26 eggs had been removed. 
February 5th, finding the deposit in peat trays No. 5 and 6 was 
great, washed both but no eggs died. February 21st, again 
washed the eggs in tray No. 2, and after 24 hours found five 
opaque ones, but no such mortality occurred in the other boxes, 
showing that although immediate injury took place no after 
effects occurred, possibly merely the weaker ones suffered. 
February 23rd, again washed the eggs in tray No. 1, where 
the total picks during the preceding ten days had merely been 
2, and they augmented to the following numbers in the next 
ten days, 2, 5, 0, 1, 1, 1, 0,0, 0,0. Consequently one has to 
decide between permitting suffocation to occur owing to parti- 
cles of sediment held in solution in the water, filling up the 
pores of the shell of the egg, or occasioning immediate loss 
from cleansing the ova. In short, although it is very evident 
that in its earliest stage (as during the first 24 hours), the 
impregnated ovum will generally stand moving, still this capa- 
bility subsequently ceases, and does not return, until at least 
one-third of the period of incubation has been completed. 
In order to still further elucidate the effects of concussion 
on these eggs further experiments were made :— 
Experiment No. 24.—January 22nd, at 10.45 a.m., droppeda 
trout egg, taken from tray 1, from a height of 2 feet, into water 
at 45°, and at 12 noon it was found to be dead and opaque. 
Experiment No. 25.—Took another egg which was dropped 
from the same height on to a board, it was then placed in an 
incubating tray, but proved to be dead at 1 p.m. 
Experiment No. 26.—January 2nd, dropped 5 eggs from a 
height of 2 feet into water, and subsequently placed them in 
