360 
211 were turned, but some appeared to be very weakly. In 
three of these fish a remarkable change had occurred in the 
colour of their fins, the ventral, anal and caudal having become 
of a carmine red. One which was 2} inches long happening to 
die, I found that its left eye had never been developed, while 
there were adhesions between the iris and subjacent structures. 
In a second the left eye had not been developed, while the right 
eye had suffered from congenital malformation. The longest 
fish was a little over 3} inches in length. On November 12th, 
1884, pond No. 3 at Howietoun was examined, and the females 
of this, termed the “zebra” race, were not quite ready for 
breeding, while they appeared to be fewer in number than the 
males, some of which were ripe. On December 24th they were 
shifted to pond No. 5, and 146 fish were present. September 
8th, 1885, the largest removed with a landing net was 94 inches 
long. November 5th, 1885, on netting pond 5, all those 
examined appeared to be sterile, the largest fish being 124 inches 
long. One of these fish was opened on November 26th, 1887, 
and found to have plenty of milt, thus showing that the cross 
between the female Lochleven trout and male American char 
will give a fertile offspring. But on November 12th, 1884, some 
eggs of the Lochleven trout were milted from one of these 
hybrids ; some of the eggs eyed, three embryos developed, but 
they died unhatched. 
The last experiment was then reversed as to the sexes of the 
parents operated upon, and on November 15th, 1882, 8,000 ova 
of an American char were fecundated with the milt of a 
Lochleven trout. They hatched in 84 days. The young fry 
were greatly deformed, many had their spines crooked, atrophy 
was present in the posterior portion of some, and a deficiency of 
the fins generally, more especially of the caudal. On March 138th, 
1884, only eight remained, which in December were shifted to 
pond 24, and in 1886 to pond 16, when on November 10th, 
1887, out of five netted none were fertile. This experiment, 
however, somewhat similar to the last, again shows that 
American char and Lochleven trout may be crossed, and that 
offspring can be raised from them. 
