96 
At the time I wrote for bugs my place was all in corn and a very large crop of chinch bugs. Iam safe in 
saying that there were more bugs on my farm than on any two farms with the same amount of land under 
cultivation. At the time of sending to you for bugs I told two of my neighbors of my intention, and 
they laughed at the idea, nevertheless I sent. When I put them in my field it had rained fully a half 
day, and after noon I commenced to place them about in different places in my field. I noticed no change 
in the bugs for three days, it being cold. On the fourth and fifth days the weather was more warm, and it 
was then that the destruction of the enemy commenced with great satistaction to myself and great surprise 
to mylaughing neighbors. One of my neighbors, Mr. George Winchester, said that there ought to be a sub- 
scription raised and donated tome. I told him not to me but to you the praise belonged. I think that 
it took about eight days after the five from the time that I placed them in my field before they were all 
destroyed. The fifth day after I put out the diseased bugs I noticed that a great many bugs were flying 
away from my place. I cannot say if the disease spread in this way or not, or if it spread at all. ree 
or four persons said they would come and procure of me some of the dead bugs, but no one came. This 
much I can say, with me this experiment has been a complete success. It has done me a great deal of good. 
I cannot give it a money value, but am satisfied that had it not been for the infected hugs obtained of you 
that I would have lost twenty-seven acres of wheat and eight acres of rye, and when I wrote to you for 
bugs I then contemplated putting out considerable wheat, and I was at that time considerably troubled 
about the bugs in my corn, thinking that if I put out any wheat at all it would be destroyed by bugs ; but 
thanks to you my wheat is now safe from bugs, at least those that were on my place before sowing my 
wheat. I only wish that I had written to you sooner than this. I will send by express one bottle of 
bugs that I gathered after they commenced to die. Respectfully yours, JoHN KNOBLE, 
The following report from R. L. Stangaard is inserted as being of a more ~ 
scientifically circumstantial character than most of the other reports: 
FLorENck, Kan., Aug. 22nd, 1890, 
Dear Sir.—In reply to your favor of July 27th, I would say that infected bugs were applied, after 
they were kept with live ones about forty-two hours. Most of the bugs mixed were dead when taken out 
of the box. They were applied in seven different hills, being put into every ninth hill. I marked every 
hill with a number so as to be better able to watch the progress. Examined after forty-eight hours ap- 
plication with the following results :—No. 1, mostly dead. No. 2, bugs mostly alive, seemingly very rest- 
less. No. 3, bugs seem to be sick. No. 4. bugs mostly dead. On hills around this one bugs seem to be 
restless. No. 5, not examined. On hills around it the bugs seem to be sick. Examination eight days 
after application with the following results :—No. 3, bugs seemingly in a dying condition. On the hills 
around it the bugs seem to be well with exception of one hill where they seem to be dying and some dead. 
No. 4, not a live bug in the hill. No.5, apparently dying, also dying in the hills around this, No. 6, bugs 
dying in hill. No.7, apparently not dying. | On August 16th, twelve days after application, I found the 
bugs to be dying and dead all through the field—twelve acres. On August 20th, I again found the bugs 
to be dying rapidly. A field being forty rods distant had sure marks of bugs in a dying condition. What I 
mean by bugs being in a dying condition is this: they lay on their backs, almost motionless, and others 
lay in same position, moving limbs violently. This remedy was applied on A. G. Rosiere’s farm on Bruno 
creek, Marion Co., Kansas, being nine miles east and three miles south of Marion. Thanking you for your 
avors, I remain, yours truly, Kk. L. StranGAARD. 
The laboratory experiments have been continued through the season, Of 
the three diseases identified, that produced by the Trichoderma appears to be 
less fatal than the other two, as is indicated by the following laboratory notes: 
September 28th, dead chinch-bugs showing no signs of fungus externally were taken from the in- 
fection jars and crushed on a glass slide in distilled water. Oval hyphal bodies of a fungus (Trichoderma) 
were found in considerable number. These were put under a bell jar. 
September 29th, some of the hyphal bodies had put out slender mycelial growths; others in im- 
mense numbers were multiplying by division. 
October Ist, the hyphal bodies were still multiplying by division. The mycelial growths had become 
much longer and in some instances had variously branched. 
October 3rd, a dead chinch-bug taken from an infected field was crushed on a glass slide in distilled 
water. Both round and oval hyphal bodies were found in considerable numbers. The sewere put undera 
bell jar to prevent dying. 
October 4th, both round and oval hypha! bodies were multiplying by division and were putting out 
mycelial growths. 
October 5th, fresh chinch-bugs from an uninfected field were immersed in the liquid containing the 
above fungi and were put in a new jar with young corn plants. 
October 16th, many of the bugs were dead ; the others apparently Bick The dead bugs were found 
to contain hyphal bodies similar to thuse with which they were infected. A live chinch-bug from the same 
jar was crushed and found to contain round hyphal bodies; but these refused to germinate. 
November 5th, not all of the bugs are yet dead. The few remaining are apparently lively. 
The following is a summary of the results of the field experiments in the 
season of 1890: 
Number of boxes of diseased bugs sent out, 38. Seven of these lots were 
either not received, or received and not used. Reports were received from 26 of 
