﻿340 
  

  

  Brown: 
  Apogamy 
  in 
  Osmunda 
  

  

  The 
  formulas 
  for 
  these 
  solutions 
  are 
  as 
  follows 
  : 
  

  

  I. 
  Prantl's 
  solution 
  (Prantl 
  '8i) 
  

  

  K2SO4 
  o.yog. 
  

  

  NaCl 
  o.23g. 
  

  

  CaSO 
  

  

  o.7og. 
  

  

  II. 
  Knop's 
  solution 
  

  

  MgSOj 
  o.25g, 
  

  

  Ca(No3)2 
  i.oog, 
  

  

  K2HPO4. 
  0.25g, 
  

  

  KCE 
  o.i2g. 
  

  

  II. 
  Knop's 
  solution 
  

  

  II 
  a, 
  Ca(N03)2 
  omitted 
  

  

  MgSOi, 
  o.sog, 
  

  

  NaaPOi 
  o.sog. 
  

  

  NH4NO3 
  solution. 
  0.064 
  per 
  cent. 
  .20 
  c.c. 
  

  

  i 
  

  

  In 
  the 
  following 
  modified 
  solutions 
  the 
  items 
  not 
  mentioned 
  

   are 
  the 
  same 
  as 
  in 
  the 
  corresponding 
  unmodified 
  solution: 
  

  

  I. 
  Pr.\ntl's 
  solution 
  

  

  I 
  a. 
  NH4NO3 
  omitted 
  

  

  I 
  6. 
  K2SO4 
  omitted 
  

  

  I 
  c, 
  NaCl 
  omitted 
  

  

  I 
  rf. 
  CaS04 
  omitted 
  

  

  I 
  e, 
  MgS04 
  omitted 
  

  

  I 
  g. 
  NaCl 
  and 
  NasPO* 
  omitted 
  

  

  After 
  the 
  spores 
  had 
  been 
  sown 
  the 
  cultures 
  were 
  placed 
  

   before 
  an 
  east 
  window 
  and 
  the 
  culture 
  solutions 
  were 
  not 
  renewed. 
  

   It 
  was 
  only 
  during 
  the 
  period 
  of 
  germination 
  that 
  the 
  cultures 
  

   were 
  exposed 
  to 
  the 
  direct 
  rays 
  of 
  the 
  sun 
  for 
  a 
  short 
  time 
  each 
  day. 
  

   If 
  left 
  longer 
  the 
  solution 
  became 
  heated 
  and 
  the 
  prothallia 
  died^ 
  

  

  Development 
  qf 
  prothallia 
  and 
  sex 
  organs 
  

  

  Germination, 
  in 
  all 
  three 
  species, 
  began 
  in 
  a 
  few 
  days 
  after 
  

   the 
  spores 
  were 
  sown. 
  

  

  O. 
  r^ga/fj.— 
  Three 
  weeks 
  later 
  the 
  prothallia 
  of 
  this 
  species 
  

   varied 
  in 
  the 
  different 
  cultures 
  from 
  four 
  to 
  fifty-nine 
  cells, 
  the 
  

  

  rregularity 
  

  

  On 
  the 
  

  

  whole 
  greater 
  growth 
  occurred 
  in 
  cultures 
  of 
  the 
  modified 
  than 
  

   in 
  those 
  of 
  the 
  unmodified 
  solutions, 
  and 
  this 
  was 
  especially 
  true 
  

   in 
  the 
  case 
  of 
  la. 
  Here 
  the 
  amount 
  of 
  nitrate 
  present 
  was 
  evi- 
  

   dently 
  sufficient 
  to 
  meet 
  the 
  needs 
  for 
  the 
  growth 
  of 
  the 
  prothallia 
  

   and 
  the 
  effect 
  of 
  the 
  unbalanced 
  solution 
  tended 
  at 
  first 
  to 
  stimu- 
  

   late 
  rather 
  than 
  retard 
  the 
  growth. 
  However, 
  the 
  reverse 
  was 
  

   true 
  in 
  the 
  later 
  development 
  of 
  the 
  prothallia, 
  for 
  at 
  the 
  end 
  of 
  

   the 
  fourth 
  month 
  in 
  lb, 
  \c, 
  Id, 
  Ig, 
  and 
  Ila 
  the 
  majority 
  of 
  the 
  

   prothallia 
  were 
  dead; 
  in 
  la, 
  I/, 
  and 
  Ig, 
  although 
  the 
  prothallia 
  

   were 
  alive, 
  growth 
  was 
  much 
  slower. 
  

  

  Campbell* 
  states, 
  ''in 
  

  

  O, 
  regalis 
  it 
  is 
  quite 
  common 
  to 
  

  

  * 
  Mosses 
  and 
  Ferns, 
  p. 
  340. 
  London. 
  1895, 
  

  

  