﻿Brown: 
  Apogamy 
  in 
  Osmunda 
  341 
  

  

  have 
  the 
  first 
  transverse 
  wall 
  followed 
  by 
  a 
  longitudinal 
  wall 
  in 
  

   each 
  cell, 
  so 
  that 
  the 
  fotir 
  primary 
  cells 
  are 
  arranged 
  quadrant- 
  

   wise.*' 
  While 
  in 
  general 
  this 
  was 
  the 
  order 
  of 
  development, 
  yet 
  

   in 
  some 
  of 
  the 
  cultures 
  a 
  filamentous 
  stage 
  of 
  two 
  and 
  three 
  cells 
  

   was 
  formed 
  before 
  longitudinal 
  division 
  took 
  place. 
  Even 
  in 
  

   those 
  cases 
  where 
  the 
  four 
  primary 
  cells 
  were 
  arranged 
  quadrant- 
  

   wise 
  the 
  prothallia 
  tended 
  to 
  assume 
  an 
  elongated 
  form 
  and 
  not 
  a 
  

   cell 
  mass 
  at 
  first. 
  In 
  Id 
  the 
  prothallia 
  were 
  very 
  broad 
  and 
  

   frequent 
  branching 
  occurred 
  at 
  the 
  expanded 
  apices 
  of 
  the 
  pro- 
  

   thallia. 
  In 
  le 
  and 
  1/ 
  the 
  growth 
  was 
  very 
  irregular, 
  some 
  of 
  the 
  

   prothallia 
  tending 
  to 
  assume 
  an 
  elongated 
  form 
  and 
  others 
  a 
  

   broad 
  form. 
  Branching 
  in 
  these 
  cultures 
  was 
  rare; 
  in 
  I 
  and 
  Ila, 
  

   however, 
  it 
  was 
  common, 
  the 
  branches 
  often 
  occurring 
  as 
  filamen- 
  

   tous 
  outgrowths 
  at 
  the 
  bases 
  of 
  the 
  young 
  prothallia, 
  which 
  were 
  

   decidedly 
  filamentous 
  in 
  form. 
  In 
  some 
  cases 
  this 
  was 
  so 
  marked 
  

   that 
  a 
  reversion 
  of 
  the 
  prothallia 
  at 
  its 
  apex 
  to 
  a 
  filamentous 
  

   condition 
  took 
  place. 
  Large 
  heart-shaped 
  prothallia, 
  monoecious 
  

   in 
  most 
  cases, 
  were 
  present 
  In 
  the 
  unmodified 
  solutions. 
  No 
  

   cases 
  of 
  apogamy 
  In 
  the 
  species 
  were 
  observed. 
  

  

  (9. 
  cinnamomea. 
  — 
  Growth 
  in 
  this 
  species 
  during 
  the 
  first 
  three 
  

   wrecks 
  was 
  not 
  so 
  rapid 
  as 
  in 
  O. 
  regalis, 
  for 
  the 
  largest 
  prothallia 
  

   were 
  only 
  thirty-four 
  cells 
  in 
  size 
  and 
  the 
  smallest 
  two 
  cells. 
  

   As 
  in 
  0. 
  regalis 
  greatest 
  growth 
  took 
  place 
  in 
  la, 
  while 
  least 
  growth 
  

   occurred 
  in 
  lb 
  and 
  I/. 
  The 
  early 
  divisions 
  of 
  the 
  prothallia 
  were 
  

  

  ■ 
  

  

  in 
  keeping 
  with 
  the 
  previously 
  quoted 
  statement 
  of 
  Campbell. 
  

   However, 
  in 
  many 
  cases 
  in 
  \a 
  the 
  filamentous 
  stage 
  consisted 
  

   of 
  from 
  three 
  to 
  five 
  cells. 
  The 
  young 
  prothallia 
  in 
  most 
  of 
  the 
  

   cultures 
  were 
  broad 
  In 
  form, 
  sometimes 
  a 
  mere 
  cell 
  mass. 
  In 
  the 
  

   case 
  of 
  the 
  prothallia 
  in 
  II 
  (Knop's 
  unmodified 
  solution), 
  while 
  the 
  

   initial 
  divisions 
  were 
  the 
  same, 
  there 
  was 
  more 
  of 
  a 
  tendency 
  for 
  

   the 
  young 
  prothallia 
  to 
  become 
  elongated 
  and 
  in 
  rare 
  cases 
  a 
  fila- 
  

   mentous 
  stage, 
  as 
  many 
  as 
  seven 
  cells 
  In 
  length, 
  was 
  formed 
  before 
  

   longitudinal 
  divisions 
  took 
  place. 
  

  

  A 
  greater 
  irregularity 
  in 
  the 
  form 
  of 
  the 
  young 
  prothallia 
  

   occurred 
  in 
  the 
  cultures 
  of 
  the 
  various 
  modified 
  solutions 
  than 
  in 
  

   the 
  same 
  cultures 
  of 
  0. 
  regalis. 
  In 
  la, 
  \c, 
  Id, 
  I/and 
  I^ 
  the 
  form 
  of 
  

   the 
  young 
  prothallia 
  was 
  very 
  irregular, 
  this 
  being 
  especially 
  

   pronounced 
  in 
  la 
  and 
  Ic. 
  Greater 
  regularity 
  of 
  form 
  occurred 
  

  

  