﻿3-i4 
  Brown: 
  Apogamy 
  in 
  Osmuxda 
  

  

  of 
  lb 
  and 
  II 
  while 
  in 
  I, 
  la, 
  Ic, 
  Id, 
  le 
  and 
  1/ 
  it 
  was 
  rare. 
  In 
  the 
  

   cultures 
  of 
  Ig 
  branching 
  of 
  the 
  prothallia 
  was 
  very 
  common. 
  

   In 
  all 
  cases 
  observed 
  the 
  branching 
  of 
  the 
  prothallia 
  occurred 
  in 
  

   the 
  younger 
  stages 
  of 
  the 
  development. 
  

  

  Sexual 
  organs 
  were 
  found 
  in 
  the 
  unmodified 
  solutions, 
  while 
  

   in 
  the 
  modified 
  solutions 
  they 
  were 
  formed 
  only 
  on 
  the 
  prothallia 
  

   in 
  la 
  and 
  lb. 
  However, 
  in 
  the 
  latter 
  only 
  a 
  few 
  sexual 
  organs 
  were 
  

   observed. 
  Normal 
  sporophytes 
  developed 
  in 
  some 
  cultures 
  of 
  

   the 
  unmodified 
  solutions 
  after 
  the 
  nutrient 
  solutions 
  had 
  been 
  

   renewed, 
  and 
  three 
  apogamous 
  outgrowths 
  occurred 
  on 
  pro- 
  

   thallia 
  in 
  lb 
  (Figs. 
  8-io). 
  

  

  Descriptions 
  of 
  the 
  apogamous 
  outgrowths 
  

  

  The 
  apogamous 
  outgrowths 
  in 
  0. 
  cinnamomea, 
  as 
  previously 
  

   stated, 
  developed 
  on 
  prothallia 
  in 
  cultures 
  of 
  I, 
  la, 
  lb 
  and 
  le. 
  One 
  

   developed 
  also 
  on 
  a 
  prothallium 
  which 
  had 
  been 
  transferred 
  from 
  I 
  

   (Prantl's 
  solution), 
  four 
  weeks 
  after 
  the 
  spores 
  were 
  sown, 
  to 
  la. 
  

  

  The 
  prothallium 
  in 
  a 
  culture 
  of 
  I 
  (Fig. 
  i), 
  upon 
  which 
  the 
  

   apogamous 
  outgrowth 
  occurred, 
  was 
  irregular 
  in 
  shape 
  and 
  

   no 
  sinus 
  was 
  present. 
  Two 
  antheridia 
  developed 
  from 
  marginal 
  

   cells, 
  one 
  in 
  the 
  expanded 
  apex 
  and 
  one 
  near 
  the 
  base 
  of 
  the 
  

   prothallium. 
  One 
  of 
  the 
  antheridia 
  was 
  stalked 
  and 
  the 
  other 
  

   sessile. 
  The 
  prothallium 
  was 
  one 
  cell 
  thick, 
  except 
  for 
  a 
  small 
  re- 
  

   gion 
  in 
  the 
  center 
  where 
  the 
  meristem 
  is 
  usually 
  formed. 
  In 
  this 
  

   region 
  a 
  swelling 
  occurred 
  which 
  continued 
  to 
  grow, 
  forming 
  a 
  

   cylindrical 
  process 
  several 
  cells 
  in 
  thickness. 
  Gradually 
  it 
  assumed 
  

   the 
  shape 
  of 
  a 
  young 
  leaf 
  and 
  finally 
  developed 
  into 
  a 
  normally 
  

   appearing 
  leaf 
  consisting 
  of 
  a 
  petiole 
  and 
  a 
  blade 
  bearing 
  a 
  forking 
  

   rib. 
  On 
  one 
  side 
  of 
  the 
  petiole 
  near 
  its 
  enlarged 
  base 
  an 
  arche- 
  

   goniura 
  which 
  appeared 
  normal 
  developed. 
  

  

  The 
  apogamous 
  outgrowth 
  which 
  occurred 
  in 
  a 
  culture 
  of 
  la 
  

   developed 
  in 
  the 
  same 
  manner 
  as 
  the 
  one 
  just 
  described. 
  This 
  

   prothallium, 
  also, 
  was 
  approximately 
  the 
  same 
  size 
  and 
  shape 
  

   (Fig. 
  2) 
  ; 
  however, 
  no 
  antheridia 
  or 
  archegonia 
  were 
  formed. 
  

  

  The 
  apogamous 
  outgrowth 
  found 
  in 
  a 
  culture 
  of 
  lb 
  developed 
  

   on 
  a 
  prothallium 
  which 
  was 
  small, 
  elongated 
  and 
  regular 
  in 
  shape. 
  

   No 
  sinus 
  was 
  present 
  and 
  from 
  the 
  merlstematic 
  region 
  near 
  the 
  

   apex 
  of 
  the 
  prothallium 
  the 
  apogamous 
  outgrowth 
  was 
  formed. 
  

   It 
  began 
  as 
  a 
  swelling 
  which 
  developed 
  into 
  a 
  cellular 
  mass. 
  

  

  