446 ALLARD: MOSAIC DISEASE OF TOBACCO 
transferring them to the field. This method, it seems, has been 
put into operation more or less generally at Deli. Experiments 
were made to determine what influence this practice had upon the 
subsequent occurrence of mosaic. The procedure was as follows: 
A mother seed bed A was prepared and sowed Feb. 17. This seed 
germinated Feb. 25. On the 14th of March, just 25 days from the 
sowing of the seed, 750 plants were transferred from A to bed B 
and set 3 x 21 inches apart. March 22, 500 plants were removed 
from bed B to bed C and set 414 x 214 inches apart. On March 
29, 250 plants were removed from bed C to bed D and set 9 x 24 
inches apart. All were fertilized alike. 
On April 2, 250 plants from beds A and B were transferred to 
the field. On April 8, 250 plants from bed C, and on April 11, 
250 plants from bed D were set in the field. 
Topping was omitted and in every way the plants were simi- 
larly treated. 
The results were as follows: Plants from the mother bed A 
were healthy and reached a height of about 214 meters. Plants 
from bed B were not as tall and were more or less mosaic. Plants 
from bed C were very inferior in every way and badly mosaic in 
most instances. Plants from bed D were not over 14 meter in 
height and not a plant escaped mosaic. It is interesting to note 
that mosaic had appeared in beds B, C and D at the time of final 
transplanting to the field. 
From these results Hunger concludes that these additional * 
transplantings have nothing to recommend them, since they 
seriously interfere with the normal growth of the plants and like- 
wise lead to the development of mosaic. 
In 1904 Bouygues and Perreau (38) stated that they obtained, 
by selection, strains of, tobacco resistant to the disease called by 
them “la nielle.” The authors refer to a previous account of 
the disease and its symptoms by Bouygues. His careful descrip- 
tion of the diseased plants makes it apparent that la nielle does 
not refer to true mosaic at all, but to some form of leaf-spot or rust. 
In the same year F. Pirazzoli (43) published an interesting 
review of the literature of mosaic. She added somewhat to the 
confusion already existing between true mosaic and various leaf- 
spot diseases, since she, like Comes, called true mosaic, ‘‘ Mal della 
bolla” and Pockenkrankheit, ‘‘mal del mosaico.”’ 
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