188 PREPARATION AND MOUNTING 
gradually add the ferrocyanide of potassium dissolved in 
another ounce, and proceed as before. 
32. Dr. Beale also gives us the following carmine injection 
to be employed in the same way as the blue.* Take— 
CDATIIIE Ws cows.» diac we se mniaitonin GERRI 5 grains. 
Glycerine, with 8 or 10 drops of liydro- 
Ghlorie ead . ce, cv dad ape sntee eae eae > ounce, 
Glycerine (pure) ss.side icici dence 1 ounce. 
PCOS) gsssvin aon snoaandacishOanataen uae aoe 2 drachms. 
WRIT. es n'seeey ncdanans dene be canes ae 6 drachms. 
Mix the carmine with a few drops of water, and when well 
incorporated add about five drops of liquor ammoniz. To 
this dark-red solution about half an ounce of the glycerine 
is to be added, and the whole well shaken in a bottle. 
Next, very gradually pour in the acid glycerine, frequently 
shaking the bottle during admixture. Test the mixture 
with blue litmus-paper, and if not of a very decidedly acid 
reaction, a few more drops of acid may be added to the 
remainder of the glycerine and mixed as before. Lastly, 
mix the alcohol and water very gradually, shaking the bottle 
thoroughly after adding each successive portion till the 
whole is mixed. This fluid may be kept ready prepared, 
and injections made very rapidly with it. 
33. The method of making injections with these colours 
is the same as with the gelatine mixtures before described, 
except that no heat is required, and consequently most of 
the trouble removed. The bottle of the fuid must be well 
shaken immediately before use; and when the object has 
been injected, we must allow it to remain in a cool place for 
a few hours before cutting it. Thin sections of the subject 
may be cut with Valentin’s knife, as before described, and 
* When, however, it is desirable to cut very thin sections of the 
injected subject, the carmine is sometimes added to a solution of fine 
gelatine—gelatine one part to water eight parts. But the warm water 
and mode of proceeding which are used with the size solutions before 
described will be necessary in this case also. 
