36 ELEMENTARY HISTOLOGY. 
b. Cells in situ: cast skin of newt. 
Take a small piece of the prepared specomen, which has been 
stained in hematoxylin, and then, after treatment with alcohol, 
cleared with creosote and turpentine. Mount the specumen in 
balsam ; cover, and examine with high power : note 
i. Flattened cells fitted together at their edges to 
form a continuous layer. Each cell has a large 
nucleus near its centre. 
II. Columnar epithelium: Consists of elongated rodlike cells 
placed vertically to the surface on which they rest. If the 
epithelium is stratified the columnar character is most marked 
in the most superficial cells. 
a, Isolated cells: From small intestine of frog: isolated 
by maceration for 24 hours in Ranvier’s alcohol, and 
stained with picro-carmine. 
Mount asmall drop of the prepared specimen in glycerine : paint 
a ring of cement round the coverglass ; and examine with high 
power ; note :— 
i. The columnar shape of the cells: their nuclei. The 
cells often remain side by side in little groups. 
b. Cells in situ. 
Take prepared section of stomach of dog which has been stained 
and then cleared in creosote and turpentine. Mount in balsam 
and examine with high power : note :-— 
i. The superficial layer of long narrow columnar cells 
packed together side by side; with nuclei at 
their inner or deeper ends. 
III. Ciliated epithelium: the cells,which are usually columnar, 
bear at their free ends tufts of exceedingly fine hair-like 
processes—cilia—which, when living, exhibit active lashing 
movements. 
a, Isolated cells. From trachea of rabbit: isolated by 
maceration for 24 hours in Ranvier’s alcohol ; stained 
with picro-carmine, and scraped into glycerine. 
Mount a small drop of the prepared specimen in glycerine : paint 
a ring of cement round the coverglass ; examine with high power, 
and note :— 
i. The shape of their cells: their nuclei; and the tuft 
of cilia at one end. 
