40 ELEMENTARY HISTOLOGY. 
II. Non-striated, or involuntary muscle. 
Take specimen of frogs bladder prepared by maceration in 
Ranvier’s alcohol for 24 hours, and removal of the epithelium of 
the inner surface by pencilling with a fine brush: stained, and 
cleared with creosote and turpentine. Mount in balsam, and 
examine with low and high powers: note :— 
i. The bands of muscular fibre. 
ii. The formation of each band by a number of 
elongated, fusiform, nucleated muscle-cells. 
ili. The absence of transverse striation in the muscle. 
D. Connective Tissues. 
Under the name “connective tissue” are included various 
tissues whose functions are mainly passive, and which serve to 
support, strengthen, and bind together the various organs and 
parts of the body. Histologically the connective tissues consist 
of elements of four kinds, united together in very varying propor- 
tions in different situations: (1) white fibrous tissue ; (2) yellow 
elastic tissue ; (3) connective tissue corpuscles, which are com- 
paratively little altered cells, usually branched ; and (4) ground 
substance, or intercellular substance. 
I, White fibrous tissue. Consists of a number of fine trans- 
parent fibres of a more or less cylindrical shape, and with 
a very characteristic wavy outline. The fibres are 
usually arranged side by side in bundles, and each fibre 
presents a number of longitudinal fibrillar striations. 
The cellular character of white fibrous tissue is difficult 
to recognise; but each fibre is formed from a single 
fusiform cell of which the nucleus disappears during 
development. 
a. Tendon of rat’s tail: pull owt a small prece of tendon 
from the tail of a rat: place it on a slide mm a drop of 
normal salt solution: spread it out with needles, cover 
and examine with high and low powers : note 
i. fibres; with wavy outlines. 
ii. fibrille: longitudinal wavy striations within the 
fibres. 
