CONNECTIVE TISSUES. 4] 
Add a drop of acetic acid to the preparation: note that 
iil. The fibres swell up and become transparent. 
iv. Rows of connective tissue corpuscles with nuclei 
become visible between the fibres. 
b. Isolated fibres and fibrille: take a small piece of tendon 
that has been macerated for 24 hours in baryta water or 
prerie acid to dissolve the cementing ground substance : 
tease it in a drop of glycerine: cover and examine with 
high power: note :— 
i. Isolated fibres and fibrille. 
II. Yellow elastic tissue. Consists of fine branched homo- 
geneous fibres, with great power of resisting chemical 
reagents: the fibres are formed from branched cells 
which lose their nuclei completely during development. 
a. Ligamentum nuche of ox: tease finely a small shred in 
water: examine with low and high powers: note :-— 
i. The branching fibres, with very sharp outlines. 
ii. The tendency of the branches to anastomose with 
one another and so form networks. 
iii, The tendency of the fibres and branches to curl up 
at their broken ends. 
Add a drop of acetic acid: note that 
iv. No alteration whatever is produced in the fibres. 
v. No nuclei appear. 
ITI. Areolar tissue: a meshwork composed of both white 
fibrous and elastic tissues. 
a. Subcutaneous tissue of mammal: Jake a freshly killed 
rat, and snip off a small piece of the loose fibrous tissue 
whach connects the skin with the subjacent parts : spread 
at on a slide: add a drop of normal salt solution: cover, 
and examine with low and high powers : note 
i. Meshwork composed of white fibrous tissue with 
wavy outlines, mingled with which are branched 
elastic fibres. 
Add acetic acid ; note that 
ii, The white fibrous tissue swells up and becomes 
transparent. 
