6 



into requisition on a number of occasions to determine actual breeding 

 points in mosquito-infested regions, and interest in the subject grad- 

 ually increased until, during the past two or three years, the researches 

 of those medical men, whose names have since become so well known 

 in this connection, showed by exact methods that Dr. King's theory 

 must no longer be considered a theory but a demonstrated fact. It 

 has resulted that the attention of the entire civilized world has been 

 drawn with vivid interest toward the whole mosquito question. Ever}^ 

 fact concerning mosquitoes becomes now of great potential importance. 

 The correspondence of this office on mosquitoes, owing largely to its 

 publications, has become greatly increased. The writer has been 

 invited to address scientific bodies and citizens' improvement associa- 

 tions on the subject of mosquito extermination, and in the spring of 

 the present year lectured before the annual meeting of the Royal 

 Society of Canada and before the section on theory and practice of 

 medicine of the American Medical Association on the subject of the 

 biology of the mosquitoes of the genus Anopheles as contrasted with 

 that of the mosquitoes of the genus Culex. The demand for the pub- 

 lications of this Division on mosquito subjects has been so great that 

 it has been deemed desirable to bring together the published and 

 unpublished articles and notes in convenient reference form from the 

 standpoint of the United States only, and this has been done in the 

 present bulletin. 



The writer is indebted to his assistants, Mr. D. W. Coquillett, for 

 determinations of the diiferent mosquitoes discussed; Mr. F. C. Pratt, 

 for untiring efforts in the collection of material; Mr. August Busck. 

 Mr. E. S. Clifton, and Mr. J. Kotinsky, for assistance in laboratory 

 exj^eriments, and Miss L. Sullivan, for the preparation of the illustra- 

 tions. Information and specimens derived from many correspondents 

 are acknowledged in the pages of the bulletin. 



L. O. H. 



