21 
(cocklebur) in the liquid and fed a large number of hoppers with it. Two infection 
boxes were started, with dampened soil covering the floor and cheese cloth the tops, 
and the insects were placed in contact with the spores of the fungus. The infected 
grasshoppers were liberated in various badly infested spots, and the results which 
should be noticed in a few weeks are looked forward to with much interest. 
In a letter to the writer dated June 5, 1900, Mr. Glenk states: 
I have had better success with the fungus than when you were here. I made an 
incubator out of some boxes and used my lamp for keeping an even temperature. 
The fungus grows well in a warm, moist atmosphere. I dissolved the nutrient agar- 
agar in two of the tubes, in hot water, mixed with it the sugar solution, added the 
fungus film, stirred well to distribute the spores, and poured the solution upon the 
leaves and moist earth. Dead grasshoppers were found in both of my infection boxes 
ina few days. I used up all the tubes received from Dr. Howard and made up 
enough liquid for all the managers. We put out a dilute solution of the fungus over 
several badly infested areas and spread it upon the grasshoppers, and the leaves of 
corn and cotton in many parts of Dahomy. 
The month of June was very propitious for the spread of disease 
among grasshoppers. Rains began the latter part of May, and during 
June 9.29 inches fell at Greenville, Miss., 40 miles south of Dahomy, 
the nearest point where meteorological records are kept. 
On July 18, 1900, the writer received the following from Mr. C. D. 
Patterson, general manager of Dahomy: 
By request of Mr. P. M. Harding I am sending you by mail to-day some dead 
grasshoppers, which we find attached to weeds on ditches and bayou banks. We 
also find a few on cotton. Mr. Ike Edwards (manager of Matthews place) tells me 
that he has found as many as six dead grasshoppers upon one stalk of cotton. The 
dead grasshoppers I am sending you were gotten on Glass place. 
Early in August a visit was made to Dahomy, with a view of making 
some observations upon the spread of this fungus, and it was found 
that over the areas where the liquid infection was spread by Mr. Glenk 
diseased hoppers were abundant. As many as a dozen dead grasshop- 
pers could be found upon a single plant, and some upon nearly every 
weed on ditch banks where grasshoppers were numerous. From the 
centers of infection great areas had become inoculated, spreading even 
beyond the plantations first infected. While a local fungus (Aimpusa 
grylli) was in evidence throughout the delta, the general spread of the 
imported fungus upon Dahomy indicates a thorough infection of this 
property with the South African fungus. The spread of the disease 
is similar to that reported from Colorado, where, Dr. Howard informs 
me, the disease has also done effective work. 
Associated with the differential locust in the same tracts of land 
were numbers of a much larger locust, the Schistocerca obscura, as well 
as many of the local species of grasshoppers found throughout the 
delta any season. Of all the specimens sent to the laboratory and of 
all those observed in the fields none were found to succumb to the 
fungus but the differential. 
