HUNGERFORD: AQUATIC HEMIPTERA. 247 
CULTURE 16168. (Oscillatoria.) 
History and Diagnosis. Pure culture of Oscillatoria obtained from 
water analysis laboratory. No animals in this culture. 
Corixids Added. Placed A. alternata here July 28. On August 1 
they were observed to work at food gathering. August 8 dissected the 
Corixids: 
One male. Digestive tract nearly empty. A brown mass of indeter- 
minate material in bend of mid-gut. 
Three females. Tracts nearly full of reddish brown material, indeter- 
minable. 
One male. As above, plus considerable number of Oscillatoria fila- 
ments, some of them still well preserved. 
CULTURE A, AuGuST 19. (Green pond ooze.) 
History and Diagnosis. Gathered some shiny, greenish ooze from the 
bottom of a mud puddle. This contains various species of Alge, especially 
the blue greens, and among the free moving Englenz and diatoms. 
Corixids Added. Several specimens of a small species of Corixid added. 
In twenty-four hours examined them. Their stomachs were packed with 
green matter. This consisted of much disorganized unicellular plant cells, 
Oscillatoria filaments so abundant that they bound the masses together, 
Englenz and diatoms. This one study would satisfy one beyond any 
question of a doubt as to the plant origin of their food. 
CULTURE B, AuGusT 19. (Water Bloom muddy.) 
History and Diagnosis Gathered some of the green scum from a stag- 
nant, muddy pool and placed it in a Petrie dish. 
It was teeming with life. Plants: Chlamydomonas, small species of 
Englena, large species Englena, diatoms, some Oscillatoria, and a few 
filaments of an alga. Englena dominant. Animals: Arcella abundant 
for this genus, Ameeba stuffed green with ingested unicellular plants. A 
few Paramecium and Heterotrichs. 
Corixids Added. A. alternata and R. acuminata placed upon this cul- 
ture. Examined following day. 3 fe) R acuminata nearly empty. An- 
other contained a small ball of yellowish green material in the bend of 
the mid intestine. This was dissected out entire and resisted separation 
as if held together by fibrous material. Most of this mass was dis- 
organized unicellular plant particles, such as noted in the Ameba. Two 
clear shafts of some filamentous algz and a membrane of some Crustacea 
and A. alternata mid-intestine packed full of green material, which re- 
sisted separation like the above. Mostly bits of greenish material, parts of 
unicellular plants, many encysted, plenty of bright green cells nearly cir- 
cular in outline. Red spots of material from disorganizing Englene. 
Two filaments of Oscillatoria, a couple of diatoms. Most material is of 
Englena origin, as shown by the fact that fresh Englene still green are 
abundant, and all stages of disorganized material are to be found. Thus 
the yellowish granular material can be traced to its unicellular plant 
origin. 
