92 JOSE F. NONIDEZ 



Figures 9 and 10 represent two early stages in the deposition 

 of the granular material. In the cell represented in figure 9 the 

 granules are extremely small and occupy the cytoplasmic vacu- 

 oles. In the hemocytoblast of figure 10 they have increased in 

 size while new ones are produced. The way in which such gran- 

 ules appear is not well known. It has been suggested that they 

 do not arise directly from the cytoplasm, but that they are 

 derived from the mitochondria (Leplat, '12), a view which is 

 supported by their disposition in rows, connected by strands 

 which stain a little more deeply than the cytoplasm. This 

 disposition is only seen in the very young granules. 



When first present the granules show a homogeneous structure, 

 staining deeply after eosin and iron hematoxylin. As growth 

 proceeds they become differentiated into two regions: a peri- 

 pheral layer slightly irregular, which strongly takes the stain, 

 and a more transparent core. This structure is conspicuous in 

 all the slides, in the stained with eosin as well as in those stained 

 with iron hematoxylin, although in the latter they generally 

 appear uniformly stained in deep black, when decolorization was 

 not pushed too far. Occasionally the granules may change into 

 rods, which are characteristic of the eosinophile leucocytes 

 in the blood-stream (Danchakoff, '08 a, b; Schmeisser, '15; 

 Kaupp, '18). 



With the deposition of granular material there appear several 

 changes in the morphology of the nucleus, which may become 

 irregular in outline from a very early stage or preserve its round 

 contour. The chromatin network stands out more clearly, 

 whereas the karyosomes tend to decrease in bulk, the plasmo- 

 somes being almost entirely deprived of the layer of chromatin 

 granules in which they lie embedded. The plasmosomes them- 

 selves shrink, eventually disappearing at later stages. 



The cells with round nucleus and granular cytoplasm or gran- 

 ulocytoblasts may increase in numbers by mitosis, during which 

 the granules are irregularly distributed among both daughter 

 cells, which preserve the same characteristics as the mother cell 

 or undergo the process which changes the granulocytoblasts into 

 granular leucocytes. 



