146 CHARLES R. STOCKARD 



When 21 days old, in lot Bi 2 more had died and 3 had hatched, 

 in B2 2 had hatched, and in B3 many had hatched. 



The control at 22 days old showed 47 hatched and 18 imhatched, 

 although all were normal. In lot Bi 5 had hatched, and 52 were 

 unhatched, the majority were normal in appearance, but 13 were 

 grossly deformed in the head region and possessed small ill-formed 

 bodies. In lot B2 4 had hatched and 36 were unhatched, of these 11 

 were grossly deformed and 25 seemed normal in structure. In lot B3 

 15 were hatched and 39 were not, of the latter 7 were grossly deformed, 

 one a typical cyclops and one a monophthalmia. Four others had 

 slightly underdeveloped eyes in addition to the 7 actually deformed. 



When 23 days old, only 2 of the control were still unhatched. In 

 lot Bi there were 35 hatched and 20 unhatched. Lot Bo contained 27 

 hatched and 12 unhatched. In lot B3 over 40 had hatched and only 

 9 were unhatched. One had died and 2 of those that had hatched 

 showed their bodies so badly twisted that they were unable to swim. 

 One of these had a badly deformed body and one eye was abnormally 

 small with the lens protruding. 



At 25 days old, every individual in the control lot had hatched. 

 Lot Bi had 16 unhatched and 4 of those that had hatched showed 

 deformed bodies and could not swim in a straightforward manner. 

 Thirty-seven of those hatched were normal in appearance, 3 of the 

 unhatched had died. The following deformed conditions existed: One 

 was a double-headed specimen, man}^ had no eyes, monophthalmia, 

 abnormally small eyes, short bodies, etc. Thus at this time after the 

 great number of specimens had died there were still over 20 per cent 

 deformed. 



In lot B2 11 were unhatched and 29 had hatched. Two of those 

 hatched were so deformed and twisted as to be unable to swim. The 

 11 unhatched ones were all grossly deformed, so there were 13, or 33 

 per cent, of the total living specimens deformed at this time. 



Lot B3 showed 5 unhatched and about 40 hatched. Four of those 

 hatched were so deformed as to be unable to swim in a normal fashion. 

 One of these presents the peculiar condition of a heart beat, but no 

 circulation in a hatched fish with a long normally shaped body. There 

 was a large accumulation of blood-cells within the sinus venosus and 

 the median vein in the region of the anus was filled with red cor- 

 puscles. This specimen could swim poorly from place to place, had 

 fairly regular respiratory movements, and waved its fins without a 

 circulation of its blood. 



When 34 days old, the Bi lot finally had 9 specimens which were 

 unable to hatch, all of them were deformed. 



Lot Bo showed 6 unable to hatch, all deformed and without a blood 

 circulation. In lot B3 4 failed to hatch. It must be recognized that 

 a great many specimens in each of the lots Bi, Bo, and B3 had died 

 during the preceding 20 days. The weaker and actually most defective 

 individuals are eliminated as shown b}^ the early mortality records. 



