Insoluble 
albuminous 
matter and 
vascular tis- 
sue. 
Extractive 
soluble in 
alcohol and 
water. 
Extractive 
soluble in 
water only. 
154 SEVENTH REPORT—1837. 
bulk of the solid matter, is to be digested on the residuum for 
half an hour ; this liquor being poured off, a second portion is to 
beadded and similarly digested; this mixture is now to be thrown 
ona filter, and washed with boiling distilled water, until the per- 
colating fluid ceases to afford a precipitation by a solution of 
nitrate of silver*. The first and second digested liquors, and 
the washings being added together, are now to be evaporated 
over a water bath till dry, and till no more weight can be lost 
by further use of the bath heat. 
We thus procure an aqueous extract C, and leave on the filter 
an insoluble residue D. The weight of extract C must be taken. 
D. The residue on the filter is now to be dried, its weight ascer- 
tained, and set down in the analysis as insoluble albuminous 
matter and vascular tissue. 
C. The aqueous extract is next to be acted upon by digestion 
for a quarter of an hour, with four times its bulk of alcohol, at 
a boiling heat. The solution so formed being poured off, a 
second portion of alcohol is to be similarly digested, the mixture 
then thrown on a filter, and the liquor allowed to percolate. The 
two portions of fluid being added together are next to be eva- 
porated to dryness over the water bath. We thus procure an 
alcoholic extract KE, and leave on the filter an extractive F, which 
is not soluble in alcohol. The former is to be dried and weighed, 
and estimated as “‘ extractive soluble in alcohol and water,” 
and the latter, similarly prepared, is to be estimated as “ extract- 
ive soluble in water only.’’ The added weights of these two 
extractives should equal that of the extract Cf. 
In conclusion, I must express my regret at having been pre- 
vented by a variety of circumstances from bringing forward ana- 
lyses of glands, either healthy or affected by some well-recog- 
nized degeneration. I have, I hope, made some amends by 
proposing a set form of examination, by the adoption of which, 
analyses, though executed by a variety of persons, may be made 
serviceable as comparative experiments in any single inquiry. 
The adoption of some such form is quite necessary before the 
objects of the Association can be answered ; for they have pro- 
posed a subject far too extensive to be developed, otherwise 
than by a multitude of experimenters, all working by the same 
rule of analysis. 
* No washings are to be commenced until all the liquor of digestion has first 
passed through the filter. 
N. B. The silver test can be used on a single drop of the filtering fluid. 
+ These extractives, as also the insoluble albuminous tissue, must be incine- 
rated, the ashes examined, and noticed in the analysis. 
7 
