TABLE III 
The effect of heat on the germination of the spores of Ascobolus carbonarius 
G9T 
No. Date No. of Medium Minutes heated Final temp Germination observed | Per cent of germi- y 
plates of oven, nation S 
: June 10 3 Goose dung agar 30 65° June 11 90+ = 
Control 4 Goose dung agar Room re) . 
2 une II 7 Heated soil agar 31 60° June 12 99 
. Control 7 Heated soil agar Room ra) = 
2 June 11 3 Goose dung agar 30 69° June 12 90+ sa 
Control 3 Goose dung agar Room ta) is) 
4 une 12, 11 A.M. I Heated soil decoction 20 70° June 12, 8 P.M. 99 iS} 
Control I Heated soil decoction Room (9) n 
¥*5 June I J decoction 20 66° Ju 90 ro) 
T6 June 15, 10 A.M. I Heated soil decoction 25 65° June 15, 5 P.M. 99 y 
vi June 15, 10 A.M I Heated soil decoction 19 60° June 15, 5 P.M. 99 fol 
8 June 21 2 Heated soil decoction 70° June 21, 11 P.M. 90+ qj 
Control I eated soil decoction Room 0 5 
9 June I Heated soil decoction 30 80° June 22, to P.M. 09 co 
10 Sept. 4 6 eated soil agar 15 70° Sept. 5 99 as 
Il Sept. 4 I Heated soil agar Room Sept. 5 0.5 
12 Sept. 27 12 eated soil agar 40 100° ft) z 
13 Sept. 28 6 Heated soil agar 35 go° Sept. 29 50 
14 Sept. 28 12 ted soil agar 20 70° Sept. 29 90+ > 
15 ot. 20 Heated soil agar 15 65° Oct. 8 90 5 
16 Oct. -7 20 Unheated carbonaceous ‘o) 
earth decoction agar 20 70 Get... & 99 % 
17 Oct. 16 2 iiss glucose agar 15 68° Oct. 21k 99 re 
18 Oct. 10 2 Malt I5 68° Oot crt 99 ~ 
19 Oct. 12 I5 Demonens n carbonaceou ie] 
earth boiled 5 min. ea 18 70° Ort; 37 99 by 
* The slide containing the spores used for no. 4 control was heated to 66° C. in the oven as sey 
y In no. 6 and 7 the temperature of the decoction was determined by stirring with a thermomet 
‘The per cent of germination was determined approximately by observing the number of ungerminated spores in given groups. 
