a ee eee ee oe mee 
DopGE: METHODS OF CULTURE OF ASCOBOLACEAE 163 
the glass slides on which they had been caught from the apothecia, 
and each slide was immersed in a beaker of the decoction of heated 
soil, which had been raised to the required temperature. In this 
manner the time of exposure was in every case five minutes. At the 
end of this time the slide was removed and placed in a Petri dish 
containing a decoction of heated soil. A fresh decoction and clean 
beaker were used in each case. As these slides were covered with 
hundreds of spores, the percentage of germination was determined 
by counting the spores visible in the field of the microscope and an 
average taken of ten countings. 
TABLE IV 
‘ Experiment to show the maximum, optimum, and minimum Pee for 
the germination of the spores of Ascobolus ade when heated 3 m 
No. of s No. of spores E 
Temperature, C ge atiaaed | not germ: stat nated Per cent germinated 
95° 0 16 0 
93° I 38 2+ 
go° 2 27 o+ 
83° 8 13 40 — 
80° 9 Io 50+ 
hi 22 o 100 
oi 7 o 100 
eg 20 § 80 
55° II 10 50+ 
45 I ey | 5 
3s” fa) 28 ° 
——ao 0 19 0 
TABLE V 
Experiment to show the maximum, optimum, and minimum temperatures for 
the germination of spores of Ascobolus States when heated 5 min. 
Temperature, C. Per cent germinated 
re 2 a ee er 0 
EEE OREN Te amine mig pn ae I 
so ER I ae Pak ead ae a ena eS 30 
Rye ec nee a a ee en a a 52 
5 9 PS ee Nol ee ee eg Re a: 90 
We ey ee, as ae es 99 
er a ee 99 
We ert s ee A el ea 84 
Wn a ee 60 
Reig ee ee a as 37 
he 3 
/ : Both series of experiments give the same general result. When 
e 
Spores are heated for three minutes we find that only five per 
