DopGE: METHODS OF CULTURE OF ASCOBOLACEAE 169 
minutes in the oven, and the oven temperature reached 70° C. 
Many of the spores germinated within 24 hours. The germ tubes 
were well developed and the hyphae formed later were not dis- 
tinguishable from the hyphae of A. immersus. Two views of a 
germinated spore are shown in FIG. 16, 26. 
TABLE VIII 
Time to Final saa tes 
seise: Date aie Medium, agar +4- noe temp. of we cesrcams' 
minutes) ven, C. 
(1910) 
Ascobolus 
Leveillei var 
mericanus Nov. 6 I j|horse dung decoction| 10 76° —_—— 
Ascobolus (1911) 
Leveillei var. 
A apex seige Det. .8 4 |horse dung decoction| 15 65° waneaeere 
ye Leveillei Apr. 7 I jhorse dung decoction) 17 68° Apr. 8 
wen May 5 I |horse dung decoction| 20 70° | May 6 
ee (1910) 
equinus Nov. 28 2 horse dung decoction| 20 80° —- 
A eae Nov. 29 I jhorse dung decoction} 11 75° Nov. 30 
us 
Aili. Nov. 15 | 1 J|horsedungdecoction| 15 ypc — 
Ascophanus Nov. 7 I {horse dung decoction| 10 70° — 
iru Dec. 20 I |horse dung decoction} 18 69° | Dec. 21 
Ascophanus (1911) 
Picea Sept. 29 3 jgoose dungdecoction| 15 74° Sept. 30 
a é 
Peels ecg Sept. 29 9 |goosedung decoction} 15 74° =| Sept. 30 
‘ : 
(ead Dec. 21 3 |N/too NasCO3;+ 
Ascobolus wood decoction 20 | 60-70° | Dec. 23 
x . 
Pa ora Dec. 19 5 |heated soil decoction! 11 gs” oe 
as Dec. 21 3. |heated soil decoction 
Thelebolus +N/50 NasCOs 17 65° | Dee. a3 
Ster 
Saccobotue” Dec. 21 2  |heated soil decoction} 20 60° | Dec. 23 
Seca Dec. 24 I |heated soil decoction) 16 65° Dec. 25 
ne 
Saban Dec. 24 I |horse dung decoction| 16 65° | Dec. 25 
neglect 
Saeco Dec. 25 I jhorse dung decoction; 20 41° Dec. 26 
neglect | 
A «d Dec. 30 I jgoosedungdecoction| 16 44° | Dec. 31 
Nigric, 
Ascodesmis Nov. 1 / 15 |goosedungdecoction|) 0 | Room | Nov. 2 
Niors 
eee | | Nov. 2| 2 WORSEN ee Batu,” bee 
