174. DopGe: METHODS OF CULTURE OF ASCOBOLACEAE 
diameter to that of the conidium itself. There is a slight con- 
striction at the point of emergence. The germ tube grows straight 
out for a distance of 50-90 u and then forms a coil (f) of two to 
four turns. The cells of this coil all have the same diameter, each 
being three or four times as long as it is thick. The entire asco- 
gonium at this stage consists of twenty to forty cells arranged in 
a loose irregular spiral tapering gradually toward the tip. The 
distal cell (g) of the stalk coil bends sharply and connects with the 
first cell (k) of the ascogonium by a large pore which is distinctly 
visible. The cell () is somewhat spherical and its diameter is 
much greater than that of the stalk cells. The distal cell (J) of 
the ascogonium grows out to form a cell (k) whose length is two 
or three timesitsdiameter. This and the ten to twenty cells next 
beyond it form an organ (1) which it seems to me must be con- 
sidered asatrichogyne. This trichogyne is more or less irregularly 
coiled and gradually tapers toward the end, which has coiled itself 
tightly about the upper part of a somewhat elongated conidium 
m). The stalk (m) of this conidium is much longer and more 
slender than the stalk of the conidium (a). From the conidiophore 
(0) there arises a stout hypha, which sweeps out in an even curve, 
extending the entire length of the ascogonial coil. It sends out 
branches at intervals (p), which may anastomose with investing 
hyphae by H-shaped connections (g), and some branches come 
to lie in the region of the end cell (7) of the ascogonium. Other 
investing hyphae arise from the cells of the stalk coil. The 
stalk coil does not taper in either direction, and the sharp contrast 
in the shape, size, and contents between the cells d and h ane 
between the cells j and & enables us to distinguish with °e 
tainty the limits of the three regions of the system, viz., the stalk, 
ascogonium, and trichogyne. The cells of these regions differ 
markedly in their behavior in fixing and staining. The cells of 
the stalk coil are dense and finely granular, are moderately 
darkened by osmic acid, and deeply and evenly stained by acet” 
carmin and safranin. The ascogonial cells have more coarsely 
granular and vacuolar contents. The cells of the trichogyne 4 
nearly hyaline, are not blackened by osmic acid, and do not staip 
readily. 
The connection between the tip of the trichogyne and the 
o- 
